A COLD-REGULATED ARABIDOPSIS GENE ENCODES A POLYPEPTIDE HAVING POTENT CRYOPROTECTIVE ACTIVITY

被引：126
|
作者：
LIN, CT
THOMASHOW, MF
机构：
[1] MICHIGAN STATE UNIV,DEPT CROP & SOIL SCI,E LANSING,MI 48824
[2] MICHIGAN STATE UNIV,DEPT MICROBIOL,E LANSING,MI 48824
[3] MICHIGAN STATE UNIV,PROGRAM GENET,E LANSING,MI 48824
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 183卷 / 03期
基金：
美国国家科学基金会;
关键词：
D O I：
10.1016/S0006-291X(05)80304-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We previously showed that cor15, a cold-regulated gene from Arabidopsis thaliana, encodes a 15 kDa polypeptide designated COR15. Here we report that COR15 has potent cryoprotective activity in an in vitro cryoprotection assay. Specifically, COR15 was very effective in protecting the cold-labile enzyme lactate dehydrogenase against freeze-inactivation; on a concentration basis, it was about 106 times more effective than sucrose and 102-103 times more effective than other proteins including bovine serum albumin. The possible role of cor15 in cold acclimation is discussed. © 1992 Academic Press, Inc.
引用
收藏
页码：1103 / 1108
页数：6
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