A COLD-REGULATED ARABIDOPSIS GENE ENCODES A POLYPEPTIDE HAVING POTENT CRYOPROTECTIVE ACTIVITY

被引：126
|
作者：
LIN, CT
THOMASHOW, MF
机构：
[1] MICHIGAN STATE UNIV,DEPT CROP & SOIL SCI,E LANSING,MI 48824
[2] MICHIGAN STATE UNIV,DEPT MICROBIOL,E LANSING,MI 48824
[3] MICHIGAN STATE UNIV,PROGRAM GENET,E LANSING,MI 48824
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 183卷 / 03期
基金：
美国国家科学基金会;
关键词：
D O I：
10.1016/S0006-291X(05)80304-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We previously showed that cor15, a cold-regulated gene from Arabidopsis thaliana, encodes a 15 kDa polypeptide designated COR15. Here we report that COR15 has potent cryoprotective activity in an in vitro cryoprotection assay. Specifically, COR15 was very effective in protecting the cold-labile enzyme lactate dehydrogenase against freeze-inactivation; on a concentration basis, it was about 106 times more effective than sucrose and 102-103 times more effective than other proteins including bovine serum albumin. The possible role of cor15 in cold acclimation is discussed. © 1992 Academic Press, Inc.
引用
收藏
页码：1103 / 1108
页数：6
相关论文
共 50 条
  • [21] Biochemical characterization of a cold-regulated protein Cor15a in Arabidopsis thaliana chloroplasts
    Nakayama, K
    Okawa, K
    Inaba, T
    PLANT AND CELL PHYSIOLOGY, 2006, 47 : S46 - S46
  • [22] A COLD-REGULATED CYANOBACTERIAL GENE-CLUSTER ENCODES RNA-BINDING PROTEIN AND RIBOSOMAL-PROTEIN S21
    SATO, N
    PLANT MOLECULAR BIOLOGY, 1994, 24 (05) : 819 - 823
  • [23] Constitutive expression of the cold-regulated Arabidopsis thaliana COR15a gene affects both chloroplast and protoplast freezing tolerance
    Artus, N. N.
    Uemura, M.
    Steponkus, P. L.
    Gilmour, S. J.
    Proceedings of the National Academy of Sciences of the United States of America, 93 (23):
  • [24] Constitutive expression of the cold-regulated Arabidopsis thaliana COR15a gene affects both chloroplast and protoplast freezing tolerance
    Artus, NN
    Uemura, M
    Steponkus, PL
    Gilmour, SJ
    Lin, CT
    Thomashow, MF
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (23) : 13404 - 13409
  • [25] Arabidopsis thaliana cold-regulated 47 gene 5′-untranslated region enables stable high-level expression of transgenes
    Yamasaki, Shotaro
    Sanada, Yuji
    Imase, Ryoji
    Matsuura, Hideyuki
    Ueno, Daishin
    Demura, Taku
    Kato, Ko
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2018, 125 (01) : 124 - 130
  • [26] CDNA SEQUENCE-ANALYSIS AND EXPRESSION OF 2 COLD-REGULATED GENES OF ARABIDOPSIS-THALIANA
    GILMOUR, SJ
    ARTUS, NN
    THOMASHOW, MF
    PLANT MOLECULAR BIOLOGY, 1992, 18 (01) : 13 - 21
  • [27] COLD-REGULATED GENE27 Integrates Signals from Light and the Circadian Clock to Promote Hypocotyl Growth in Arabidopsis[OPEN]
    Zhu, Wei
    Zhou, Hua
    Lin, Fang
    Zhao, Xianhai
    Jiang, Yan
    Xu, Dongqing
    Deng, Xing Wang
    PLANT CELL, 2020, 32 (10): : 3155 - 3169
  • [28] A mitochondrial complex I defect impairs cold-regulated nuclear gene expression
    Lee, BH
    Lee, HJ
    Xiong, LM
    Zhu, JK
    PLANT CELL, 2002, 14 (06): : 1235 - 1251
  • [29] A novel cold-regulated gene from Phlox subulata, PsCor413im1, enhances low temperature tolerance in Arabidopsis
    Zhou, Aimin
    Sun, Hongwei
    Feng, Shuang
    Zhou, Mi
    Gong, Shufang
    Wang, Jingang
    Zhang, Shuzhen
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 495 (02) : 1688 - 1694
  • [30] Transcriptional adaptor and histone acetyltransferase proteins in Arabidopsis and their interactions with CBF1, a transcriptional activator involved in cold-regulated gene expression
    Stockinger, EJ
    Mao, YP
    Regier, MK
    Triezenberg, SJ
    Thomashow, MF
    NUCLEIC ACIDS RESEARCH, 2001, 29 (07) : 1524 - 1533
12345