Improved Methods to Produce Tissue-Engineered Skin Substitutes Suitable for the Permanent Closure of Full-Thickness Skin Injuries

被引：43

作者：

Larouche, Danielle ^{[1
,2
]}

Cantin-Warren, Laurence ^{[1
,2
]}

Desgagne, Maxime ^{[1
,2
]}

Guignard, Rina ^{[1
,2
]}

Martel, Israel ^{[1
,2
]}

Ayoub, Akram ^{[1
,2
]}

Lavoie, Amelie ^{[1
,2
]}

Gauvin, Robert ^{[1
,2
,3
]}

Auger, Francois A. ^{[1
,2
]}

Moulin, Veronique J. ^{[1
,2
]}

Germain, Lucie ^{[1
,2
]}

机构：

[1] Univ Laval, Ctr Rech Organogenese Expt, Fac Med, Dept Chirurg,LOEX, Quebec City, PQ, Canada

[2] Univ Laval, CHU Quebec, Ctr Rech, Axe Med Regeneratrice, Quebec City, PQ, Canada

[3] CQMF, Quebec City, PQ, Canada

来源：

BIORESEARCH OPEN ACCESS | 2016年 / 5卷 / 01期

基金：

加拿大健康研究院;

关键词：

autologous; regenerative medicine; skin equivalent; tissue culture; tissue therapy; transplants;

D O I：

10.1089/biores.2016.0036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

There is a clinical need for skin substitutes to replace full-thickness skin loss. Our group has developed a bilayered skin substitute produced from the patient's own fibroblasts and keratinocytes referred to as Self-Assembled Skin Substitute (SASS). After cell isolation and expansion, the current time required to produce SASS is 45 days. We aimed to optimize the manufacturing process to standardize the production of SASS and to reduce production time. The new approach consisted in seeding keratinocytes on a fibroblast-derived tissue sheet before its detachment from the culture plate. Four days following keratinocyte seeding, the resulting tissue was stacked on two fibroblast-derived tissue sheets and cultured at the air-liquid interface for 10 days. The resulting total production time was 31 days. An alternative method adapted to more contractile fibroblasts was also developed. It consisted in adding a peripheral frame before seeding fibroblasts in the culture plate. SASSs produced by both new methods shared similar histology, contractile behavior in vitro and in vivo evolution after grafting onto mice when compared with SASSs produced by the 45-day standard method. In conclusion, the new approach for the production of high-quality human skin substitutes should allow an earlier autologous grafting for the treatment of severely burned patients.

引用

页码：320 / 329

页数：10