DETECTION OF THE ENZYMATIC-ACTIVITY OF CYTOCHROME-P-450 ENZYMES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：10
作者：
JANSEN, EHJM
DEFLUITER, P
机构：
[1] Laboratory for Toxicology, National Institute of Public Health and Environmental Protection, 3720 BA Bilthoven
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 580卷 / 1-2期
关键词：
D O I：
10.1016/0378-4347(92)80541-W
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The reactions catalysed by the various cytochrome P-450 enzymes are reviewed with respect to the analysis of products by high-performance liquid chromatography (HPLC). Especially biotransformation reactions of purified cytochrome P-450 enzymes in a reconstituted system and in microsomes mainly of rat liver origin are considered. Emphasis is put on the specificity of product formation due to the individual isozymes of cytochrome P-450. It is shown that the presence of eight cytochrome P-450 isozymes can be monitored and determined by specific product formation after HPLC analysis, which is an important parameter in toxicological studies.
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页码：325 / 346
页数：22
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