THE FATE OF MITOCHONDRIAL DNAS OF MT+ AND MT- ORIGIN IN GAMETES AND ZYGOTES OF CHLAMYDOMONAS

被引：18

作者：

BECKERS, MC ^{[1
]}

MUNAUT, C ^{[1
]}

MINET, A ^{[1
]}

MATAGNE, RF ^{[1
]}

机构：

[1] UNIV LIEGE,DEPT BOT B22,B-4000 LIEGE,BELGIUM

来源：

CURRENT GENETICS | 1991年 / 20卷 / 03期

关键词：

MITOCHONDRIAL DNA; UNIPARENTAL INHERITANCE; CHLAMYDOMONAS;

D O I：

10.1007/BF00326238

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In order to study the mechanism responsible for the uniparental transmission of the mitochondrial genome in crosses between Chlamydomonas reinhardtii and C. smithii, we have analyzed the fate of mitochondrial DNA during gametogenesis, zygospore differentiation and sporulation by hybridization experiments. Both mt+ and mt- gametes contain the same amount of mitochondrial DNA and the two parental genomes persist for several days in the zygotes. The DNA of mt+ origin is slowly eliminated during the period of zygote maturation. Light is required for total elimination of mt+ mitochondrial DNA in the zygospores. Using appropriate restriction enzymes, we have been unable to detect methylation of the mitochondrial DNA during gametogenesis or zygospore formation. The possibility that the mt+ mitochondria themselves are specifically eliminated in the course of zygote maturation is discussed.

引用

页码：239 / 243

页数：5

共 21 条

[1] MITOCHONDRIAL GENOME OF CHLAMYDOMONAS - INDUCTION OF MINUTE COLONY MUTATIONS BY ACRIFLAVINE AND THEIR INHERITANCE [J].