PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF THE OMEGA-CONOTOXIN-SENSITIVE N-TYPE CALCIUM-CHANNEL FROM RABBIT BRAIN

被引：220

作者：

FUJITA, Y

MYNLIEFF, M

DIRKSEN, RT

KIM, MS

NIIDOME, T

NAKAI, J

FRIEDRICH, T

IWABE, N

MIYATA, T

FURUICHI, T

FURUTAMA, D

MIKOSHIBA, K

MORI, Y

BEAM, KG

机构：

[1] KYOTO UNIV, FAC MED, DEPT MOLEC GENET, KYOTO 60601, JAPAN

[2] COLORADO STATE UNIV, COLL VET MED & BIOMED SCI, DEPT PHYSIOL, FT COLLINS, CO 80523 USA

[3] KYOTO UNIV, FAC SCI, DEPT BIOPHYS, KYOTO 60601, JAPAN

[4] UNIV TOKYO, INST MED SCI, DEPT MOLEC NEUROBIOL, TOKYO 108, JAPAN

来源：

NEURON | 1993年 / 10卷 / 04期

关键词：

D O I：

10.1016/0896-6273(93)90162-K

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The complete amino acid sequence of a rabbit brain calcium channel (BIII) has been deduced by cloning and sequencing the cDNA. The open reading frame encodes 2339 amino acids, which corresponds to an M(r) of 261,167. A phylogenetic tree representing evolutionary relationships indicates that BIII is grouped together with the other rabbit brain calcium channels, BI and BII, into a subfamily that is distinct from the dihydropyridine-sensitive L-type subfamily. Transient expression in cultured skeletal muscle myotubes derived from muscular dysgenic mice demonstrates that the BIII channel mediates an omega-conotoxin-sensitive calcium current with kinetics and voltage dependence like those previously reported for whole-cell N-type current. Cell-attached patch recordings, with isotonic barium as the charge carrier, revealed distinct single channels with an average slope conductance of 14.3 pS.

引用

页码：585 / 598

页数：14

共 82 条

[61] A MUTANT OF TTX-RESISTANT CARDIAC SODIUM-CHANNELS WITH TTX-SENSITIVE PROPERTIES [J].