HETEROLOGOUS GENE-EXPRESSION ON THE LINEAR DNA KILLER PLASMID FROM KLUYVEROMYCES-LACTIS

被引：47
|
作者：
KAMPER, J
ESSER, K
GUNGE, N
MEINHARDT, F
机构：
[1] UNIV MUNSTER, INST MIKROBIOL, CORRENSSTR 3, W-4400 MUNSTER, GERMANY
[2] KUMAMOTO INST TECHNOL, DEPT APPL MICROBIAL TECHNOL, KUMAMOTO 860, JAPAN
[3] RUHR UNIV BOCHUM, LEHRSTUHL ALLGEMEINE BOT, W-4630 BOCHUM 1, GERMANY
来源：
CURRENT GENETICS | 1991年 / 19卷 / 02期
关键词：
LINEAR PLASMIDS; KILLER; GENE EXPRESSION;
D O I：
10.1007/BF00326291
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Linear hybrid plasmids based on the killer plasmid pGKL1 from Kluyveromyces lactis were obtained by in vivo recombination in Saccharomyces cerevisiae. Like pGKL1, the hybrids are located in the cytoplasm, have terminal inverted repeats (TIR) and possess covalently linked proteins at their 5' ends. The construction of cytoplasmic hybrid plasmids is based on the use of a pGKL1 promoter to control the marker gene used for recombination. Nuclear promoters are not recognised in the cytoplasm.
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页码：109 / 118
页数：10
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