DETERMINATION OF LACTATE AND LACTIC-DEHYDROGENASE BY BIAMPEROMETRIC MONITORING OF HEXACYANOFERRATE(II) IN A FLOWING STREAM

被引：2

作者：

ATTIYAT, AS

CHRISTIAN, GD

机构：

[1] Department of Chemistry, University of Washington, Seattle, 98195, WA

来源：

FRESENIUS ZEITSCHRIFT FUR ANALYTISCHE CHEMIE | 1979年 / 295卷 / 2-3期

关键词：

Best. von Lactat; Lactatdehydrogenase; Biamperometrie; Hexacyanoferrat(II); Durchfluß; Kohlenstoffelektrode;

D O I：

10.1007/BF01171503

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Open tubular carbon electrodes were used to monitor the biamperometric current of hexacyanoferrate(II) ion produced by the LDH catalyzed reaction between hexacyanoferrate(III) ion and lactate, and that produced from the diaphorase catalyzed reaction between hexacyanoferrate(III) ion and NADH, product from the LDH catalyzed reaction between lactate and NAD. Reagents and samples were mixed in a flow stream. Reaction takes place between the mixing point and the electrodes, and measurements are taken after a constant time interval. Lactate and LDH standards in serum control solutions were measured using the NAD dependent reaction. © 1979 Springer-Verlag.

引用

页码：157 / 160

页数：4

共 22 条

[1]

ALDERMAN JA, 1977, CLIN CHEM, V23, P1917

[2]

ATTIYAT AS, UNPUBLISHED

[3] STUDY OF A REAGENTLESS LACTATE ELECTRODE [J].