A STUDY OF BIOSYNTHESIS OF DEOXYRIBOSE IN WHEAT EMBRYOS

被引:3
作者
FAN, ML
LACROIX, LJ
UNRAU, AM
机构
[1] Department of Plant Science, University of Manitoba, Winnipeg
[2] Department of Chemistry, Simon Fraser University, Burnaby
关键词
D O I
10.1021/bi00838a027
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Glucose labeled in various carbons with 14C was fed to wheat embryos for four different time periods. Deoxyribose was isolated and sequentially degraded to determine the position of labeling. When glucose-6-14C was supplied, radioactivity first appeared in C5 while longer feeding periods resulted in some label incorporation in C3 followed by random labeling of C1, C2, and C4. Embryo feeding with glucose-2-14C resulted in radioactivity appearing in C1, C2, and C4 with randomization at long feeding periods. Feeding glucose-1-14C resulted in label appearing first in Q and C5 followed by C3 and random labeling of C2 and C4. On the basis of these results, it is suggested that a combination of the oxidative and nonoxidative pathways is involved. © 1969, American Chemical Society. All rights reserved.
引用
收藏
页码:4083 / &
相关论文
共 18 条
[1]  
COLTER JS, 1962, BIOCHIM BIOPHYS ACTA, V55, P31
[2]   BIOSYNTHESIS OF RIBOSE AND DEOXYRIBOSE IN PSEUDOMONAS SACCHAROPHILA [J].
FOSSITT, DD ;
BERNSTEIN, IA .
JOURNAL OF BACTERIOLOGY, 1963, 86 (06) :1326-&
[3]  
GHOSH D, 1963, BIOCHIM BIOPHYS ACTA, V72, P19
[4]  
HAMMARSTEN E, 1950, J BIOL CHEM, V183, P105
[5]   SUGAR NUCLEOTIDES IN THE INTERCONVERSION OF CARBOHYDRATES IN HIGHER PLANTS [J].
HASSID, WZ ;
NEUFELD, EF ;
FEINGOLD, DS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1959, 45 (07) :905-915
[6]  
HIATT HH, 1958, J BIOL CHEM, V233, P1023
[7]  
HOLLMANN S, 1964, NONGLYCOLYTIC PATHWA, P115
[8]  
HOLLMANN S, 1956, J AM CHEM SOC, V78, P3544
[9]   A COMPARISON OF C-14-LABELING PATTERNS IN DEOXYRIBOSE AND RIBOSE IN MAMMALIAN CELLS [J].
HORECKER, BL ;
DOMAGK, G ;
HIATT, HH .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1958, 78 (02) :510-517
[10]   MASS ISOLATION OF VIABLE WHEAT EMBRYOS [J].
JOHNSTON, FB ;
STERN, H .
NATURE, 1957, 179 (4551) :160-161