Establishment of vasopressin-responsive early proximal tubular cell lines derived from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene

被引：0

作者：

Takeda, M

Hosoyamada, M

Shirato, I

Obinata, M

Suzuki, M

Endou, H

机构：

[1] JUNTENDO UNIV,SCH MED,DEPT MED,DIV NEPHROL,TOKYO 113,JAPAN

[2] TOHOKU UNIV,SCH MED,DEPT CELL BIOL,TB & CANC RES INST,SENDAI,MIYAGI 980,JAPAN

[3] KUMAMOTO UNIV,SCH MED,INST MOL EMBRYOL & GENET,KUMAMOTO 860,JAPAN

来源：

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1995年 / 37卷 / 03期

关键词：

vasopressin receptor; SV; 40; proximal tubule; calcium; cell line;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A little is known about vasopressin receptor in early proximal tubule (S1). The purpose of this study is to establish the vasopressin-responsive S1 cell line derived from transgenic mice harboring temperature-sensitive(ts) simian virus (SV) 40 large T-antigen gene. The cells showed a temperature-sensitive cell growth characteristic of encoding tsSV40. The S1 cells retained a unique morphology specific to proximal tubule. The cells showed the vasopressin-induced increase in intracellular calcium concentration ([Ca2+]i) mediated by both V1a and the putative Vp receptor. Of nineteen clonal cell lines established from the parental cells, three expressed only V1a receptor, and five retained both V1a and Vp receptor In conclusion, these immortalized S1 cell lines may be useful for studying vasopressin receptor subtypes in S1.

引用

页码：507 / 515

页数：9

共 17 条

[1] RECEPTORS FOR NEUROHYPOPHYSEAL HORMONES ALONG THE RAT NEPHRON - I-125 LABELED D(CH2)5[TYR(ME)2, THR4, ORN8, TYR-NH2(9)] VASOTOCIN BINDING IN MICRODISSECTED TUBULES [J].