DETECTION AND LOCALIZATION OF INTERLEUKIN-8 MESSENGER-RNA AND PROTEIN IN HUMAN PLACENTA AND DECIDUAL TISSUES

被引：44

作者：

SAITO, S ^{[1
]}

KASAHARA, T ^{[1
]}

SAKAKURA, S ^{[1
]}

UMEKAGE, H ^{[1
]}

HARADA, N ^{[1
]}

ICHIJO, M ^{[1
]}

机构：

[1] JICHI MED SCH, DEPT MED BIOL & PARASITOL, MINAMI KAWACHI, TOCHIGI 32904, JAPAN

来源：

JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 1994年 / 27卷 / 03期

关键词：

IL-8; HUMAN PLACENTA; DECIDUA; ENDOMETRIAL GLAND; IN SITU HYBRIDIZATION;

D O I：

10.1016/0165-0378(94)90001-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

It has been reported that interleukin-8 (IL-8) is secreted from the placental and decidual tissues and that IL-8 levels in the amniotic fluids are significantly elevated by chorioamnionitis or labor pain. The present study was aimed at defining the localization of IL-8 mRNA as well as IL-8 protein at the feto-maternal interface using in situ hybridization and immunohistochemical staining. Both IL-8 mRNA and protein were localized in cytotrophoblast, syncytiotrophoblast and Hofbauer cells of the placenta, decidual stromal cells, decidual lymphocytes and endometrial gland cells. IL-8 secretion from glandular cells has not previously been reported. In addition, we confirmed IL-8 mRNA expression and secretion of IL-8 by an endometrial cancer cell line (Ishikawa) using the reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) methods, respectively.

引用

页码：161 / 172

页数：12

共 34 条

[1] LOCALIZATION OF LEUKOCYTES AND CYTOKINES IN CHORIONIC VILLI OF NORMAL PLACENTAS AND COMPLETE HYDATIDIFORM MOLES [J].

BERKOWITZ, RS ;

FARIS, HMP ;

HILL, JA ;

ANDERSON, DJ .

GYNECOLOGIC ONCOLOGY, 1990, 37 (03) :396-400

[2] EXPRESSION OF COLONY-STIMULATING FACTOR-I IN THE HUMAN UTERUS AND PLACENTA [J].

DAITER, E ;

PAMPFER, S ;

YEUNG, YG ;

BARAD, D ;

STANLEY, ER ;

POLLARD, JW .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1992, 74 (04) :850-858

[3]

DJEU JY, 1990, J IMMUNOL, V144, P2205

[4] INFLAMMATORY MEDIATORS REGULATE INTERLEUKIN-8 PRODUCTION BY CULTURED GESTATIONAL TISSUES - EVIDENCE FOR A CYTOKINE NETWORK AT THE CHORIO-DECIDUAL INTERFACE [J].

DUDLEY, DJ ;

TRAUTMAN, MS ;

MITCHELL, MD .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1993, 76 (02) :404-410

[5] TUMOR-NECROSIS-FACTOR-ALPHA MESSENGER-RIBONUCLEIC-ACID AND PROTEIN IN HUMAN ENDOMETRIUM [J].

HUNT, JS ;

CHEN, HL ;

HU, XL ;

TABIBZADEH, S .

BIOLOGY OF REPRODUCTION, 1992, 47 (01) :141-147

[6]

KASAHARA T, 1991, IMMUNOLOGY, V74, P60

[7] CHORIODECIDUAL PRODUCTION OF INTERLEUKIN-8 AND MECHANISM OF PARTURITION [J].

KELLY, RW ;

LEASK, R ;

CALDER, AA .

LANCET, 1992, 339 (8796) :776-777

[8] A SENSITIVE ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR HUMAN INTERLEUKIN-8 [J].

KO, YC ;

MUKAIDA, N ;

PANYUTICH, A ;

VOITENOK, NN ;

MATSUSHIMA, K ;

KAWAI, T ;

KASAHARA, T .

JOURNAL OF IMMUNOLOGICAL METHODS, 1992, 149 (02) :227-235

[9] ELEVATED INTERLEUKIN-8 LEVELS IN THE URINE OF PATIENTS WITH URINARY-TRACT INFECTIONS [J].

KO, YC ;

MUKAIDA, N ;

ISHIYAMA, S ;

TOKUE, A ;

KAWAI, T ;

MATSUSHIMA, K ;

KASAHARA, T .

INFECTION AND IMMUNITY, 1993, 61 (04) :1307-1314

[10]

KO YC, 1994, IN PRESS J CLIN PATH

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