COMPLETE AMINO-ACID-SEQUENCE OF PHENOMYCIN, AN ANTITUMOR POLYPEPTIDE ANTIBIOTIC

被引：4

作者：

MURAMATSU, R ^{[1
]}

ABE, S ^{[1
]}

HAYASHI, H ^{[1
]}

YAMAGUCHI, K ^{[1
]}

JINDA, K ^{[1
]}

SAKANO, K ^{[1
]}

INOUYE, Y ^{[1
]}

NAKAMURA, S ^{[1
]}

机构：

[1] NIPPON MIN CO LTD,DEPT PHARMACEUT,BIOSCI RES LABS,TODA,SAITAMA,JAPAN

来源：

JOURNAL OF ANTIBIOTICS | 1991年 / 44卷 / 11期

关键词：

D O I：

10.7164/antibiotics.44.1222

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The primary structure of phenomycin, an antitumor polypeptide antibiotic isolated from the culture broth of Streptomyes fervens var. phenomyceticus, was determined. Sequence analysis has revealed that it consists of 89 amino acid residues (M(r) 9,524) and no disulfide bridge is present. The sequence of phenomycin at residues 48 approximately 70 was found to be hydrophilic, being surrounded by hydrophobic regions at both sides. Furthermore, clustering of half the lysine residues in this hydrophilic region and marked basicity of phenomycin imply the external disposition of this region. Phenomycin does not show significant sequence homology to any known proteins including antitumor polypeptide antibiotics.

引用

页码：1222 / 1227

页数：6

共 14 条

[1] AMINO-ACID-ANALYSIS AT THE PICOMOLE LEVEL - APPLICATION TO THE C-TERMINAL SEQUENCE-ANALYSIS OF POLYPEPTIDES
CHANG, JY
KNECHT, R
BRAUN, DG
[J]. BIOCHEMICAL JOURNAL, 1981, 199 (03) : 547 - 555
[2] Chou P Y, 1978, Adv Enzymol Relat Areas Mol Biol, V47, P45
[3] Hoop TP, 1985, P NATL ACAD SCI USA, V78, P3824
[4] ISHIDA N, 1965, J ANTIBIOT, V18, P68
[5] SECONDARY STRUCTURES OF PROTEINS AND PEPTIDES IN AMPHIPHILIC ENVIRONMENTS (A REVIEW)
KAISER, ET
KEZDY, FJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-PHYSICAL SCIENCES, 1983, 80 (04): : 1137 - 1143
[6] ROLES OF CHROMOPHORE AND APOPROTEIN IN NEOCARZINOSTATIN ACTION
KAPPEN, LS
NAPIER, MA
GOLDBERG, IH
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (04): : 1970 - 1974
[7] CHROMOPHORIC LABELING OF AMINO-ACIDS WITH 4-DIMETHYLAMINOAZOBENZENE-4'-SULFONYL CHLORIDE
LIN, JK
CHANG, JY
[J]. ANALYTICAL CHEMISTRY, 1975, 47 (09) : 1634 - 1638
[8] MAEDA H, 1966, J ANTIBIOT, V19, P253
[9] NAKAMURA S, 1967, J ANTIBIOT, V20, P210
[10] ACTIVITY OF PHENOMYCIN AGAINST TRANSPLANTABLE ANIMAL TUMORS
NISHIMURA, T
[J]. JOURNAL OF ANTIBIOTICS, 1968, 21 (02) : 106 - +

← 1 2 →