EXPRESSION AND SECRETION OF BOVINE BETA-LACTOGLOBULIN IN SACCHAROMYCES-CEREVISIAE

被引：28

作者：

TOTSUKA, M ^{[1
]}

KATAKURA, Y ^{[1
]}

SHIMIZU, M ^{[1
]}

KUMAGAI, I ^{[1
]}

MIURA, K ^{[1
]}

KAMINOGAWA, S ^{[1
]}

机构：

[1] UNIV TOKYO,FAC ENGN,DEPT IND CHEM,BUNKYO KU,TOKYO 113,JAPAN

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1990年 / 54卷 / 12期

关键词：

D O I：

10.1080/00021369.1990.10870482

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A bovine beta-lactoglobulin (beta-LG) was expressed in Saccharomyces cerevisiae carrying bovine pre-beta-LG cDNA and secreted into its growth medium. The expression plasmid was constructed by inserting the whole coding region of the cDNA encoding pre-beta-LG between the promoter and terminator of the yeast glyceraldehyde 3-phosphate dehydrogenase gene of pYG100, a yeast expression vector. In the supernatant of the yeast growth medium, beta-LG with a native conformation was detected by sandwich ELISA, and its amount was estimated to be 1.1 mg/l. A Western-immunoblotting analysis revealed that-beta-LG secreted in the growth medium had the same mobility as that of authentic bovine-beta-LG. The N-terminal sequence was also identical with that of authentic mature bovine-beta-LG.

引用

页码：3111 / 3116

页数：6