EBNA1 DISTORTS ORIP, THE EPSTEIN-BARR-VIRUS LATENT REPLICATION ORIGIN

被引：66

作者：

FRAPPIER, L ^{[1
]}

ODONNELL, M ^{[1
]}

机构：

[1] CORNELL UNIV,MED CTR,COLL MED,HOWARD HUGHES MED INST,DEPT MICROBIOL,1300 YORK AVE,NEW YORK,NY 10021

来源：

JOURNAL OF VIROLOGY | 1992年 / 66卷 / 03期

关键词：

D O I：

10.1128/JVI.66.3.1786-1790.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Epstein-Barr virus nuclear antigen 1 (EBNA1) protein binds and activates the latent replication origin (oriP) of the Epstein-Barr virus. We have been studying EBNA1 to determine how it activates replication at oriP. Here we demonstrate that upon binding of EBNA1 to oriP, two thymine residues become reactive to potassium permanganate (KMnO4), indicating a helical distortion at these sites. The KMnO4-reactive thymines are 64 bp apart in the region of dyad symmetry of oriP. Dimethyl sulfate protection studies indicated that EBNA1 binds on the opposite face of the helix from the reactive thymines. The nature of the helical distortion induced by EBNA1 and its possible significance to the initiation of replication are discussed.

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页码：1786 / 1790

页数：5

相关论文

共 35 条

[1] REPLICATION OF LATENT EPSTEIN-BARR-VIRUS GENOMES IN RAJI CELLS [J].

ADAMS, A .

JOURNAL OF VIROLOGY, 1987, 61 (05) :1743-1746

[2] DEFINITION OF THE SEQUENCE REQUIREMENTS FOR BINDING OF THE EBNA-1 PROTEIN TO ITS PALINDROMIC TARGET SITES IN EPSTEIN-BARR-VIRUS DNA [J].

AMBINDER, RF ;

SHAH, WA ;

RAWLINS, DR ;

HAYWARD, GS ;

HAYWARD, SD .

JOURNAL OF VIROLOGY, 1990, 64 (05) :2369-2379

[3] LOCALIZED MELTING AND STRUCTURAL-CHANGES IN THE SV40 ORIGIN OF REPLICATION INDUCED BY T-ANTIGEN [J].

BOROWIEC, JA ;

HURWITZ, J .

EMBO JOURNAL, 1988, 7 (10) :3149-3158

[4] DIFFERENTIAL INDUCTION OF STRUCTURAL-CHANGES IN THE SIMIAN VIRUS-40 ORIGIN OF REPLICATION BY T-ANTIGEN [J].

BOROWIEC, JA ;

DEAN, FB ;

HURWITZ, J .

JOURNAL OF VIROLOGY, 1991, 65 (03) :1228-1235

[5] HIGH-RESOLUTION ANALYSIS OF LAC TRANSCRIPTION COMPLEXES INSIDE CELLS [J].

BOROWIEC, JA ;

GRALLA, JD .

BIOCHEMISTRY, 1986, 25 (18) :5051-5057

[6] DNA SUPERCOILING PROMOTES FORMATION OF A BENT REPRESSION LOOP IN LAC DNA [J].

BOROWIEC, JA ;

LI, Z ;

SASSEDWIGHT, S ;

GRALLA, JD .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 196 (01) :101-111

[7] DUPLEX OPENING BY DNAA PROTEIN AT NOVEL SEQUENCES IN INITIATION OF REPLICATION AT THE ORIGIN OF THE ESCHERICHIA-COLI CHROMOSOME [J].

BRAMHILL, D ;

KORNBERG, A .

CELL, 1988, 52 (05) :743-755

[8] FUNCTIONAL LIMITS OF ORIP, THE EPSTEIN-BARR VIRUS PLASMID ORIGIN OF REPLICATION [J].

CHITTENDEN, T ;

LUPTON, S ;

LEVINE, AJ .

JOURNAL OF VIROLOGY, 1989, 63 (07) :3016-3025

[9]

DEAN FB, 1991, J BIOL CHEM, V266, P5062

[10] SPECIALIZED NUCLEOPROTEIN STRUCTURES AT THE ORIGIN OF REPLICATION OF BACTERIOPHAGE-LAMBDA - LOCALIZED UNWINDING OF DUPLEX DNA BY A 6-PROTEIN REACTION [J].

DODSON, M ;

ECHOLS, H ;

WICKNER, S ;

ALFANO, C ;

MENSAWILMOT, K ;

GOMES, B ;

LEBOWITZ, J ;

ROBERTS, JD ;

MCMACKEN, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (20) :7638-7642

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