A GANCICLOVIR-RESISTANT CLINICAL ISOLATE OF HUMAN CYTOMEGALOVIRUS EXHIBITING CROSS-RESISTANCE TO OTHER DNA-POLYMERASE INHIBITORS

被引：76

作者：

TATAROWICZ, WA

LURAIN, NS

THOMPSON, KD

机构：

[1] LOYOLA UNIV,MED CTR,DEPT PATHOL,MAYWOOD,IL 60153

[2] LOYOLA UNIV,MED CTR,DEPT MICROBIOL,MAYWOOD,IL 60153

来源：

JOURNAL OF INFECTIOUS DISEASES | 1992年 / 166卷 / 04期

关键词：

D O I：

10.1093/infdis/166.4.904

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Clinical isolates of human cytomegalovirus (HCMV) were screened for susceptibility to ganciclovir by plaque-reduction assay and in situ ELISA. A pretreatment isolate of HCMV obtained from the bronchial brushing of a heart transplant recipient contained both ganciclovir-susceptible and -resistant virus. Ganciclovir-susceptible (P8) and -resistant (D16) strains were further isolated by plaque purification. Both strains phosphorylated ganciclovir at levels similar to the ganciclovir-susceptible strain AD169. D16 was also resistant to phosphonoformic acid and to (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)adenine and cytosine. These data suggest that the resistance of D16 to these drugs results from a mutation in the viral DNA polymerase gene.

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页码：904 / 907

页数：4

相关论文

共 16 条

[1] A HUMAN CYTOMEGALOVIRUS MUTANT RESISTANT TO THE NUCLEOSIDE ANALOG 9-([2-HYDROXY-1-(HYDROXYMETHYL)ETHOXY]METHYL)GUANINE (BW B759U) INDUCES REDUCED LEVELS OF BW B759U TRIPHOSPHATE [J].

BIRON, KK ;

FYFE, JA ;

STANAT, SC ;

LESLIE, LK ;

SORRELL, JB ;

LAMBE, CU ;

COEN, DM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (22) :8769-8773

[2] PREVALENCE OF RESISTANCE IN PATIENTS RECEIVING GANCICLOVIR FOR SERIOUS CYTOMEGALOVIRUS-INFECTION [J].

DREW, WL ;

MINER, RC ;

BUSCH, DF ;

FOLLANSBEE, SE ;

GULLETT, J ;

MEHALKO, SG ;

GORDON, SM ;

OWEN, WF ;

MATTHEWS, TR ;

BUHLES, WC ;

DEARMOND, B .

JOURNAL OF INFECTIOUS DISEASES, 1991, 163 (04) :716-719

[3] PROGRESSIVE DISEASE DUE TO GANCICLOVIR-RESISTANT CYTOMEGALO-VIRUS IN IMMUNOCOMPROMISED PATIENTS [J].

ERICE, A ;

CHOU, S ;

BIRON, KK ;

STANAT, SC ;

BALFOUR, HH ;

JORDAN, MC .

NEW ENGLAND JOURNAL OF MEDICINE, 1989, 320 (05) :289-293

[4] ACQUISITION OF CYTOMEGALO-VIRUS INFECTION - AN UPDATE [J].

FORBES, BA .

CLINICAL MICROBIOLOGY REVIEWS, 1989, 2 (02) :204-216

[5] IDENTIFICATION OF AMINO-ACIDS IN HERPES-SIMPLEX VIRUS-DNA POLYMERASE INVOLVED IN SUBSTRATE AND DRUG RECOGNITION [J].

GIBBS, JS ;

CHIOU, HC ;

BASTOW, KF ;

CHENG, YC ;

COEN, DM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (18) :6672-6676

[6] FOSCARNET THERAPY FOR GANCICLOVIR-RESISTANT CYTOMEGALOVIRUS RETINITIS IN PATIENTS WITH AIDS [J].

JACOBSON, MA ;

DREW, WL ;

FEINBERG, J ;

ODONNELL, JJ ;

WHITMORE, PV ;

MINER, RD ;

PARENTI, D .

JOURNAL OF INFECTIOUS DISEASES, 1991, 163 (06) :1348-1351

[7] SEQUENCE AND TRANSCRIPTION ANALYSIS OF THE HUMAN CYTOMEGALOVIRUS DNA-POLYMERASE GENE [J].

KOUZARIDES, T ;

BANKIER, AT ;

SATCHWELL, SC ;

WESTON, K ;

TOMLINSON, P ;

BARRELL, BG .

JOURNAL OF VIROLOGY, 1987, 61 (01) :125-133

[8] ENGINEERED HERPES-SIMPLEX VIRUS-DNA POLYMERASE POINT MUTANTS - THE MOST HIGHLY CONSERVED REGION SHARED AMONG ALPHA-LIKE DNA-POLYMERASES IS INVOLVED IN SUBSTRATE RECOGNITION [J].

MARCY, AI ;

HWANG, CBC ;

RUFFNER, KL ;

COEN, DM .

JOURNAL OF VIROLOGY, 1990, 64 (12) :5883-5890

[9]

MATTHEWS T, 1988, REV INFECT DIS, V10, pS490

[10] ANTIVIRAL EFFECTS OF PHOSPHONOFORMATE (PFA, FOSCARNET SODIUM) [J].

OBERG, B .

PHARMACOLOGY & THERAPEUTICS, 1989, 40 (02) :213-285