FLUOROMETRIC-DETERMINATION OF ISONIAZID AND ITS METABOLITES IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING IN-LINE DERIVATIZATION

被引：7
|
作者：
KOHNO, H
KUBO, H
FURUKAWA, K
YOSHINO, N
NISHIKAWA, T
机构：
[1] HOSP MASAGO,NATL SANATORIUM,DIV PHARMACEUT,NIIGATA,JAPAN
[2] KITASATO UNIV,SCH MED,KANAGAWA,JAPAN
[3] KITASATO UNIV,SCH PHARMACEUT SCI,TOKYO 108,JAPAN
来源：
THERAPEUTIC DRUG MONITORING | 1991年 / 13卷 / 05期
关键词：
REVERSED-PHASE ION-EXCHANGE CHROMATOGRAPHY; ISONIAZID METABOLITES IN URINE; FLUOROMETRIC DETECTION; ACETYLATOR PHENOTYPING;
D O I：
10.1097/00007691-199109000-00007
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
A rapid, simple, and accurate method has been developed for the determination of isoniazid and its metabolites (isonicotinic acid, isonicotinylglycine, and acetylisoniazid) in human urine by high-performance liquid chromatography. Isoniazid and its metabolites are separated by reversed-phase ion-exchange chromatography with a mobile phase containing hydrogen peroxide as a fluorogenic reagent and butanesulfonate as a hydrophobic ion exchanger, and are detected by fluorometry (excitation at 317 nm and emission at 415 nm) using in-line derivatization at high temperature (160-degrees-C). The detection limits are isonicotinic acid, 0.5-mu-mol/L; isonicotinylglycine, 1-mu-mol/L; acetylisoniazid, 1-mu-mol/L; and isoniazid, 1.5-mu-mol/L. This method can be applied for acetylator phenotyping.
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页码：428 / 432
页数：5
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