Purification and Characterization of Extracellular alpha-amylase from Aspergillus niger K8

被引：0
作者：
Emara, H. M. [1 ]
Reyad, A. M. [2 ]
机构：
[1] Benha Univ, Fac Sci, Bot Dept, Banha, Egypt
[2] Beni Suef Univ, Fac Sci, Dept Bot, Bani Suwayf, Egypt
来源：
EGYPTIAN JOURNAL OF BOTANY | 2014年 / 54卷 / 01期
关键词：
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
ASPERGILLUS NIGER K8 was selected from twenty-two fungal isolates for alpha-Amylase production. The crude enzyme was partially purified and precipitated by salting out with ammonium sulphate. The fraction salted out with 60% ammonium sulphate saturation showed the highest specific alpha-amylase activity with 5.42 folds of purification than that of free extract. The optimum temperature for alpha-amylase enzyme activity was at 60 degrees C. The enzyme was very stable at 60 degrees C for 1 hr and retained more than 80% of its original activity. The optimum specific activity for A. niger K8 was in a narrow range of pH 5.0-6.0. Alpha amylase enzyme was stable at all pH tested. However, it retains more than 75% of its original activity for 1 h at pH value 5.5.
引用
收藏
页码：1 / 11
页数：11
相关论文
共 50 条
[41]   Extracellular phytase from Aspergillus niger CFR 335: purification and characterization [J].
Gunashree, B.S. ;
Venkateswaran, G. .
Journal of Food Science and Technology, 2015, 52 (07) :4558-4564
[42]   Effects of Aspergillus niger (K8) on nutritive value of rice straw [J].
Jahromi, M. F. ;
Liang, J. B. ;
Rosfarizan, M. ;
Goh, Y. M. ;
Shokryazdan, P. ;
Ho, Y. W. .
AFRICAN JOURNAL OF BIOTECHNOLOGY, 2010, 9 (42) :7043-7047
[43]   Purification, biochemical characterization and application of alpha-amylase produced by Aspergillus oryzae IFO-30103 [J].
Dey, Tapati Bhanja ;
Banerjee, Rintu .
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2015, 4 (01) :83-90
[44]   PRODUCTION, PURIFICATION, AND CHARACTERIZATION OF ALPHA-AMYLASE FROM THERMOMONOSPORA-CURVATA [J].
GLYMPH, JL ;
STUTZENBERGER, FJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1977, 34 (04) :391-397
[45]   PURIFICATION AND CHARACTERIZATION OF A THERMOSTABLE ALPHA-AMYLASE FROM THERMOACTINOMYCES-VULGARIS [J].
HEESE, O ;
HANSEN, G ;
HOHNE, WE ;
KORNER, D .
BIOMEDICA BIOCHIMICA ACTA, 1991, 50 (03) :225-232
[46]   PURIFICATION AND CHARACTERIZATION OF A THERMOSTABLE ALPHA-AMYLASE FROM BACILLUS-LICHENIFORMIS [J].
IVANOVA, VN ;
DOBREVA, EP ;
EMANUILOVA, EI .
JOURNAL OF BIOTECHNOLOGY, 1993, 28 (2-3) :277-289
[47]   PURIFICATION AND PROPERTIES OF ALPHA-AMYLASE FROM ASPERGILLUS-ORYZAE ATCC-76080 [J].
CHANG, CT ;
TANG, MS ;
LIN, CF .
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, 1995, 36 (01) :185-193
[48]   Production and characterization of α-amylase from Aspergillus niger [J].
M.J.P. Rohilkhand University, Bareilly, U.P., India ;
不详 ;
不详 .
Biotechnology, 2008, 3 (551-556) :551-556
[49]   OSTRICH ALPHA-AMYLASE - PURIFICATION AND CHARACTERIZATION OF THE PANCREATIC ISOENZYMES [J].
OOSTHUIZEN, V ;
NAUDE, RJ ;
OELOFSEN, W .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1994, 26 (06) :833-841
[50]   PURIFICATION AND CHARACTERIZATION OF EXTRACELLULAR ALPHA-AMYLASE AND GLUCOAMYLASE FROM THE YEAST CANDIDA-ANTARCTICA CBS-6678 [J].
DEMOT, R ;
VERACHTERT, H .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 164 (03) :643-654
12345