Purification and Characterization of Extracellular alpha-amylase from Aspergillus niger K8

被引：0
作者：
Emara, H. M. [1 ]
Reyad, A. M. [2 ]
机构：
[1] Benha Univ, Fac Sci, Bot Dept, Banha, Egypt
[2] Beni Suef Univ, Fac Sci, Dept Bot, Bani Suwayf, Egypt
来源：
EGYPTIAN JOURNAL OF BOTANY | 2014年 / 54卷 / 01期
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中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
ASPERGILLUS NIGER K8 was selected from twenty-two fungal isolates for alpha-Amylase production. The crude enzyme was partially purified and precipitated by salting out with ammonium sulphate. The fraction salted out with 60% ammonium sulphate saturation showed the highest specific alpha-amylase activity with 5.42 folds of purification than that of free extract. The optimum temperature for alpha-amylase enzyme activity was at 60 degrees C. The enzyme was very stable at 60 degrees C for 1 hr and retained more than 80% of its original activity. The optimum specific activity for A. niger K8 was in a narrow range of pH 5.0-6.0. Alpha amylase enzyme was stable at all pH tested. However, it retains more than 75% of its original activity for 1 h at pH value 5.5.
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页码：1 / 11
页数：11
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