SIMULTANEOUS DETECTION OF 2 MESSENGER-RNAS IN THE CENTRAL-NERVOUS-SYSTEM - A SIMPLE 2-STEP INSITU HYBRIDIZATION PROCEDURE USING A COMBINATION OF RADIOACTIVE AND NONRADIOACTIVE PROBES

被引：26

作者：

NORMAND, E ^{[1
]}

BLOCH, B ^{[1
]}

机构：

[1] UNIV BORDEAUX 2,HISTOL EMBRYOL LAB,CNRS,URA 1200,146 RUE LEO SAIGNAT,F-33076 BORDEAUX,FRANCE

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1991年 / 39卷 / 11期

关键词：

INSITU HYBRIDIZATION; MESSENGER RNA; COLOCALIZATION; NEUROPEPTIDE; RECEPTOR; CENTRAL NERVOUS SYSTEM;

D O I：

10.1177/39.11.1918932

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We present here a method enabling the simultaneous detection of two messenger RNAs in tissue sections by use of a two-step in situ hybridization procedure. Tissue sections were hybridized with a radioactive probe and coated with emulsion. The emulsion was processed for development, fixed, and a second hybridization was performed through the emulsion with a biotinylated probe subsequently revealed with streptavidin-alkaline phosphatase. This procedure allows the detection of two mRNAs without loss of signal, removal of the emulsion, or spurious reaction. The simultaneous detection of oxytocin and vasopressin mRNAs in the hypothalamus, and of dopamine receptor and neuropeptide mRNAs in the striatum, demonstrated the efficiency of the procedure. Such a two-step procedure provides a simple and flexible way to make possible comparative analysis of the localization of two mRNAs within the same tissue section.

引用

页码：1575 / 1578

页数：4

共 26 条

[21] DISTRIBUTION OF ALPHA-2, ALPHA-3, ALPHA-4, AND BETA-2 NEURONAL NICOTINIC RECEPTOR SUBUNIT MESSENGER-RNAS IN THE CENTRAL NERVOUS-SYSTEM - A HYBRIDIZATION HISTOCHEMICAL-STUDY IN THE RAT
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WADA, K
BOULTER, J
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HEINEMANN, S
PATRICK, J
SWANSON, LW
JOURNAL OF COMPARATIVE NEUROLOGY, 1989, 284 (02) : 314 - 335
[22] STAGE-SPECIFIC EXPRESSION OF RAT TRANSITION PROTEIN 2 MESSENGER-RNA AND POSSIBLE LOCALIZATION TO THE CHROMATID BODY OF STEP-7 SPERMATIDS BY INSITU HYBRIDIZATION USING A NONRADIOACTIVE RIBOPROBE
SAUNDERS, PTK
MILLAR, MR
MAGUIRE, SM
SHARPE, RM
MOLECULAR REPRODUCTION AND DEVELOPMENT, 1992, 33 (04) : 385 - 391
[23] MOLECULAR-CLONING OF IL-2R-ALPHA, IL-2R-BETA, AND IL-2R-GAMMA CDNAS FROM A HUMAN OLIGODENDROGLIOMA CELL-LINE - PRESENCE OF IL-2R MESSENGER-RNAS IN THE HUMAN CENTRAL-NERVOUS-SYSTEM
OTERO, GC
MERRILL, JE
GLIA, 1995, 14 (04) : 295 - 302
[24] LIGHT-MICROSCOPIC DETECTION OF INTER-ALPHA-TRYPSIN INHIBITOR AND ITS DIFFERENT MESSENGER-RNAS IN CULTURED HEPATOMA HEP G2 CELLS USING IMMUNOCYTOCHEMICAL AND IN-SITU HYBRIDIZATION TECHNIQUES
BORGHI, H
CALLE, A
SESBOUE, R
BOURGUIGNON, J
DIARRAMEHRPOUR, M
MARTIN, JP
HISTOCHEMICAL JOURNAL, 1994, 26 (03): : 252 - 261
[25] QUANTITATION OF THE MESSENGER-RNAS CODING FOR B-CELL AND T-CELL SPECIFIC RECEPTOR PROTEINS IN LEUKEMIAS OF DIFFERENT MATURATION STAGES BY INSITU HYBRIDIZATION WITH FLUOROCHROME-LABELED GENE PROBES .2. EXPRESSION OF RECEPTOR GENE RNAS IN T-DETERMINATED LEUKEMIAS AND ABERRANT EXPRESSION IN LEUKEMIAS CARRYING MARKERS OF NONLYMPHOID LINEAGE AFFILIATIONE
PACHMANN, K
MAR, P
REINECKE, K
DORKEN, B
EMMERICH, B
THIEL, E
BLUT, 1987, 55 (04): : 292 - 292
[26] DETECTION OF GLIOMA-ASSOCIATED GANGLIOSIDES GM2, GD2, GD3, 3'-ISOLM1 AND 3',6'-ISOLD1 IN CENTRAL-NERVOUS-SYSTEM TUMORS IN-VITRO AND IN-VIVO USING EPITOPE-DEFINED MONOCLONAL-ANTIBODIES
WIKSTRAND, CJ
FREDMAN, P
SVENNERHOLM, L
BIGNER, DD
BIOLOGICAL FUNCTION OF GANGLIOSIDES: PROCEEDINGS OF NOBEL SYMPOSIUM 83, 1994, 101 : 213 - 223

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