CLONING AND EXPRESSION OF PECTIN LYASE GENE FROM ERWINIA-CAROTOVORA IN ESCHERICHIA-COLI

被引：8

作者：

NISHIDA, T

SUZUKI, T

ITO, K

KAMIO, Y

IZAKI, K

机构：

[1] Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University, Sendai

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)92392-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A pectin lyase (PNL; EC 4.2.2.10) gene of Erwinia carotovora Er was cloned and expressed in Escherichia coli. The analysis of the nucleotide sequence of the 0.6 kb StuI-EcoRI fragment, which was hybridized with the mixed oligonucleotide probe for PNL gene, revealed the presence of an open reading frame (ORF) and correlated exactly with the known N-terminal 18 amino acid sequence of PNL. When a plasmid pTN2159, which has a BamHI-EcoRI fragment containing this ORF, was introduced into E. coli JM109, PNL was not expressed. When a tac-promoter was inserted in front of the ORF, PNL was efficiently expressed in E. coli. Synthesis of PNL by E. coli was also confirmed by immunoblot analysis. © 1990.

引用

页码：801 / 808

页数：8

共 17 条

[1] GENETICS OF ERWINIA SPECIES [J].