CONVERSION OF [14C]CYCLOARTENOL AND [14C]LANOSTEROL INTO PHYTOSTEROLS BY CULTURES OF NICOTIANA TABACUM

被引：50
作者：
HEWLINS, MJE
EHRHARDT, JD
HIRTH, L
OURISSON, G
机构：
[1] Institut de Chimie Université de Strasbourg Esplanade, Strasbourg
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 8卷 / 02期
关键词：
D O I：
10.1111/j.1432-1033.1969.tb00513.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Tissue cultures of Nicotiana tabacum were treated with [14C]cyeloartenol and [14C]lanosterol, and the 4,4‐dimethylsterol 4‐methylsterol and phytosterol fractions were examined for radioactivity. In both cases activity was incorporated into the phytosterols. The distributions of radioactivity in the 4,4‐dimethylsterols and 4‐methylsterols were compared with those obtained by incorporation of [14C]acetic acid. It is concluded that cycloartenol is a true phytosterol precursor in the tissue cultures, whereas the possible role of lanosterol (not detectable in the cultures, even by isotopic dilution experiments) is not proven. Copyright © 1969, Wiley Blackwell. All rights reserved
引用
收藏
页码：184 / &
相关论文
共 14 条
[1]   PHYTOSTEROL BIOSYNTHESIS FROM LANOSTEROL IN EUPHORBIA PEPLUS [J].
BAISTED, DJ ;
GARDNER, RL ;
MCREYNOLDS, LA .
PHYTOCHEMISTRY, 1968, 7 (06) :945-+
[2]  
BENNET RD, 1965, PHYTOCHEMISTRY, V7, P775
[3]   LA BIOSYNTHESE DES STEROLS DANS LES TISSUS DE TABAC CULTIVES IN VITRO .2. PARTICULARITES DE LA BIOSYNTHESE DES PHYTOSTEROLS DES TISSUS DE TABAC CULTIVES IN VITRO [J].
BENVENISTE, P ;
HIRTH, L ;
OURISSON, G .
PHYTOCHEMISTRY, 1966, 5 (01) :45-+
[4]  
BENVENISTE P, UNPUBLISHED WORK
[5]  
BENVENISTE P, 1967, THESIS STRASBOURG
[6]   ALPHA-SPINASTEROL BIOSYNTHESIS FROM 24-METHYLENE DIHYDROLANOSTEROL IN SPINACEA OLERACEA LEAVES [J].
DEVYS, M ;
ALCAIDE, A ;
BARBIER, M ;
LEDERER, E .
PHYTOCHEMISTRY, 1968, 7 (04) :613-+
[7]   ETUDES SUR LES TRITERPENES PRECURSEURS DES PHYTOSTEROLS . RECHERCHE DU CYCLOARTENOL ET DU LANOSTEROL DANS DIVERSES ESPECES VEGETALES [J].
EHRHARDT, JD ;
HIRTH, L ;
OURISSON, G .
PHYTOCHEMISTRY, 1967, 6 (06) :815-&
[8]   ANEROBIC CYCLIZATION OF SQUALENE-2,3-EPOXIDES TO CYCLOARTENOL IN NICOTIANA TABACUM L TISSUE CULTURES [J].
EPPENBER.U ;
HIRTH, L ;
OURISSON, G .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1969, 8 (02) :180-&
[9]  
HIRTH L, 1958, THESIS PARIS
[10]   STUDIES ON PHYTOSTEROL BIOSYNTHESIS - OBSERVATIONS ON ESTERIFIED STEROLS OF HIGHER PLANTS [J].
KEMP, RJ ;
HAMMAM, ASA ;
GOAD, LJ ;
GOODWIN, TW .
PHYTOCHEMISTRY, 1968, 7 (03) :447-&
12