CLONING AND SEQUENCING OF A GENE ENCODING PYRUVATE-KINASE FROM SCHIZOSACCHAROMYCES-POMBE - IMPLICATIONS FOR QUATERNARY STRUCTURE AND REGULATION OF THE ENZYME

被引：1
作者：
NAIRN, J
SMITH, S
ALLISON, PJ
RIGDEN, D
FOTHERGILLGILMORE, LA
PRICE, NC
机构：
[1] UNIV STIRLING,DEPT BIOL & MOLEC SCI,STIRLING FK9 4LA,SCOTLAND
[2] UNIV EDINBURGH,DEPT BIOCHEM,EDINBURGH EH8 9XD,MIDLOTHIAN,SCOTLAND
来源：
FEMS MICROBIOLOGY LETTERS | 1995年 / 134卷 / 2-3期
基金：
英国惠康基金;
关键词：
PYRUVATE KINASE; SCHIZOSACCHAROMYCES POMBE; SUBUNIT INTERFACE;
D O I：
10.1016/0378-1097(95)00408-3
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A cDNA encoding pyruvate kinase from Schizosaccharomyces pombe has been isolated from a lambda ZAPII library. This cDNA was sequenced and found to contain an open reading frame of 1524 nucleotides, giving a predicted protein subunit M(r) of 55470. The sequence shows a high degree of identity with other pyruvate kinase sequences, with residues implicated in the binding of substrate and metal ion co-factors conserved. However, there are significant differences in the putative subunit interface and effector binding regions which may account for the unusual quaternary structure and regulatory properties of the S. pombe enzyme.
引用
收藏
页码：221 / 226
页数：6
相关论文
共 17 条
  • [1] A SUBUNIT INTERFACE MUTANT OF YEAST PYRUVATE-KINASE REQUIRES THE ALLOSTERIC ACTIVATOR FRUCTOSE-1,6-BISPHOSPHATE FOR ACTIVITY
    COLLINS, RA
    MCNALLY, T
    FOTHERGILLGILMORE, LA
    MUIRHEAD, H
    [J]. BIOCHEMICAL JOURNAL, 1995, 310 : 117 - 123
  • [2] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX
    DEVEREUX, J
    HAEBERLI, P
    SMITHIES, O
    [J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (01) : 387 - 395
  • [3] PROPERTIES OF PYRUVATE-KINASE FROM SCHIZOSACCHAROMYCES-POMBE
    DUNCAN, D
    MCGOWAN, J
    PRICE, NC
    [J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 1989, 17 (03) : 560 - 561
  • [4] FOTHERGILLGILMO.LA, 1993, PROG BIOPHYS MOL BIO, V59, P105
  • [5] A CDNA FROM SCHIZOSACCHAROMYCES-POMBE ENCODING A PUTATIVE ENOLASE
    JACKSON, JC
    LOPES, JM
    [J]. GENE, 1995, 154 (01) : 109 - 113
  • [6] STRUCTURE OF RABBIT MUSCLE PYRUVATE-KINASE COMPLEXED WITH MN2+, K+, AND PYRUVATE
    LARSEN, TM
    LAUGHLIN, LT
    HOLDEN, HM
    RAYMENT, I
    REED, GH
    [J]. BIOCHEMISTRY, 1994, 33 (20) : 6301 - 6309
  • [7] CRYSTAL-STRUCTURE OF ESCHERICHIA-COLI PYRUVATE-KINASE TYPE-I - MOLECULAR-BASIS OF THE ALLOSTERIC TRANSITION
    MATTEVI, A
    VALENTINI, G
    RIZZI, M
    SPERANZA, ML
    BOLOGNESI, M
    CODA, A
    [J]. STRUCTURE, 1995, 3 (07) : 729 - 741
  • [8] THE YEAST PYRUVATE-KINASE GENE DOES NOT CONTAIN A STRING OF NON-PREFERRED CODONS - REVISED NUCLEOTIDE-SEQUENCE
    MCNALLY, T
    PURVIS, IJ
    FOTHERGILLGILMORE, LA
    BROWN, AJP
    [J]. FEBS LETTERS, 1989, 247 (02) : 312 - 316
  • [9] THE STRUCTURE OF CAT MUSCLE PYRUVATE-KINASE
    MUIRHEAD, H
    CLAYDEN, DA
    BARFORD, D
    LORIMER, CG
    FOTHERGILLGILMORE, LA
    SCHILTZ, E
    SCHMITT, W
    [J]. EMBO JOURNAL, 1986, 5 (03) : 475 - 481
  • [10] THE AMINO-ACID-SEQUENCE OF THE SMALL MONOMERIC PHOSPHOGLYCERATE MUTASE FROM THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE
    NAIRN, J
    PRICE, NC
    FOTHERGILLGILMORE, LA
    WALKER, GE
    FOTHERGILL, JE
    DUNBAR, B
    [J]. BIOCHEMICAL JOURNAL, 1994, 297 : 603 - 608
12