MOLECULAR AND GENETIC-CHARACTERIZATION OF ELITE TRANSGENIC RICE PLANTS PRODUCED BY ELECTRIC-DISCHARGE PARTICLE-ACCELERATION

被引：137

作者：

COOLEY, J ^{[1
]}

FORD, T ^{[1
]}

CHRISTOU, P ^{[1
]}

机构：

[1] AGRACETUS INC,MIDDLETON,WI 53562

来源：

THEORETICAL AND APPLIED GENETICS | 1995年 / 90卷 / 01期

关键词：

TRANSGENIC RICE; ORYZA SATIVA; COEXPRESSION; PARTICLE BOMBARDMENT; HERBICIDE RESISTANCE;

D O I：

10.1007/BF00221001

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The recovery of transgenic rice plants expressing a number of exogenous genes was reported previously. Using immature embryo explants as the target tissue, plasmids containing both selectable and screenable marker genes were introduced into elite rice varieties via electric-discharge particle acceleration. Co-integration, copy number, expression, and inheritance of these genes were analyzed. A 100% co-integration frequency was confirmed by Southern-blot analyses of R0 plants. The majority of transgenic plants contained between one and ten copies of exogenous DNA and molecular and genetic analyses of progeny indicated that all copies in almost all RO plants were inherited as a single dominant hemizygous locus. Co-expression of unselected genes ranged from 30-66% for gus/hmr constructs, depending on the promotor used, and up to 90% for bar/hmr constructs. The integrative structures of two unlinked transgenic loci of a rare R0 plant were analyzed in detail by Southern-blot analysis of its progeny.

引用

页码：97 / 104

页数：8

共 40 条

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