EFFECTS OF VANADATE ON 6-PHOSPHOFRUCTO 2-KINASE ACTIVITY AND FRUCTOSE 2,6-BISPHOSPHATE LEVELS IN CULTURED RAT HEPATOCYTES

被引：5

作者：

MIRALPEIX, M

KATZ, NR

BARTRONS, R

机构：

[1] UNIV BARCELONA,FAC ODONTOL,DEPT CIENCIES FISIOL HUMANES & NUTR,UNITAT BIOQUIM,E-08907 LHOSPITALET,SPAIN

[2] UNIV FREIBURG,MED KLIN,W-7800 FREIBURG,GERMANY

来源：

CELL BIOCHEMISTRY AND FUNCTION | 1990年 / 8卷 / 04期

关键词：

6‐phosphofructo; 2‐kinase; cultured rat hepatocytes; Fructose 2,6‐bisphosphate; vanadate;

D O I：

10.1002/cbf.290080409

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of vanadate in primary cultures of rat hepatocytes produced a significant increase in the concentration of fructose 2,6‐bisphosphate and in the activity of 6‐phosphofructo 2‐kinase. Compared with insulin, vanadate had a more potent action on the metabolite increase, but a similar effect on the 6‐phosphofructo 2‐kinase activity. Both the insulin‐ and the vanadate‐dependent enhancements of 6‐phosphofructo 2‐kinase were inhibited by cycloheximide which specificially blocks protein synthesis on the translational level, suggesting that the increase of the enzyme activity was due to induction rather than to a change in the catalytic activity. Copyright © 1990 John Wiley & Sons Ltd.

引用

页码：237 / 241

页数：5

共 50 条

[1] REGULATION OF THE PHOSPHORYLATION AND ACTIVITY OF 6-PHOSPHOFRUCTO 1-KINASE IN ISOLATED HEPATOCYTES BY ALPHA-GLYCEROLPHOSPHATE AND FRUCTOSE 2,6-BISPHOSPHATE
CLAUS, TH
SCHLUMPF, JR
ELMAGHRABI, MR
PILKIS, SJ
JOURNAL OF BIOLOGICAL CHEMISTRY, 1982, 257 (13) : 7541 - 7548
[2] GLUCAGON-INDUCED CHANGES IN FRUCTOSE 2,6-BISPHOSPHATE AND 6-PHOSPHOFRUCTO-2-KINASE IN CULTURED RAT FETAL HEPATOCYTES
MARTINSANZ, P
CASCALES, M
BOSCA, L
BIOCHEMICAL JOURNAL, 1989, 257 (03) : 795 - 799
[3] RAT HEPATIC 6-PHOSPHOFRUCTO 2-KINASE FRUCTOSE 2,6-BISPHOSPHATASE - A UNIQUE BIFUNCTIONAL ENZYME
PILKIS, SJ
CHRISMAN, T
BURGRESS, B
MCGRANE, M
COLOSIA, A
PILKIS, J
CLAUS, TH
ELMAGHRABI, MR
ADVANCES IN ENZYME REGULATION, 1983, 21 : 147 - +
[4] MECHANISM OF CATALYSIS OF RAT-LIVER 6-PHOSPHOFRUCTO 2-KINASE FRUCTOSE 2,6-BISPHOSPHATASE
ELMAGHRABI, MR
REGEN, DM
PILKIS, SJ
FEDERATION PROCEEDINGS, 1983, 42 (07) : 2080 - 2080
[5] PRIMARY SEQUENCE OF HEPATIC 6-PHOSPHOFRUCTO 2-KINASE FRUCTOSE 2,6-BISPHOSPHATASE
COLOSIA, AD
ELMAGHRABI, MR
PILKIS, SJ
LIVELY, MO
FEDERATION PROCEEDINGS, 1987, 46 (06) : 2228 - 2228
[6] ISOLATION OF A CDNA CLONE FOR RAT-LIVER 6-PHOSPHOFRUCTO 2-KINASE FRUCTOSE 2,6-BISPHOSPHATASE
COLOSIA, AD
LIVELY, M
ELMAGHRABI, MR
PILKIS, SJ
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 143 (03) : 1092 - 1098
[7] THE EFFECT OF FRUCTOSE ON FRUCTOSE 2,6-BISPHOSPHATE LEVEL AND FRUCTOSE 6-PHOSPHATE, 2-KINASE ACTIVITY IN THE PERFUSED-RAT-LIVER
OGAWA, A
NISHI, T
FURUYA, E
WATANABE, F
SAKAI, A
KIDO, Y
TSUJINAKA, T
MORI, T
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, 1993, 30 (01): : 83 - 90
[8] ACTIVATION OF GLYCOLYSIS BY INSULIN WITH A SEQUENTIAL INCREASE OF THE 6-PHOSPHOFRUCTO-2-KINASE ACTIVITY, FRUCTOSE-2,6-BISPHOSPHATE LEVEL AND PYRUVATE-KINASE ACTIVITY IN CULTURED RAT HEPATOCYTES
PROBST, I
UNTHANFECHNER, K
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 153 (02): : 347 - 353
[9] CHARACTERIZATION OF THE EXCHANGE-REACTIONS OF RAT-LIVER 6-PHOSPHOFRUCTO 2-KINASE/FRUCTOSE 2,6-BISPHOSPHATASE
ELMAGHRABI, MR
PATE, TM
PILKIS, SJ
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 123 (02) : 749 - 756
[10] 6-PHOSPHOFRUCTO 2-KINASE/FRUCTOSE 2,6-BISPHOSPHATASE IN LIVER OF THE TELEOST SPARUS-AURATA
DE FRUTOS, PG
BAANANTE, IV
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1994, 308 (02) : 461 - 468

← 1 2 3 4 5 →