DYNAMICS OF STRUCTURAL-CHANGES IN BIOLOGICAL PARTICLES FROM RAPID LIGHT-SCATTERING MEASUREMENTS

被引:3
|
作者
MORRIS, SJ [1 ]
SHULTENS, HA [1 ]
HELLWEG, MA [1 ]
STRIKER, G [1 ]
JOVIN, TM [1 ]
机构
[1] MAX PLANCK INST BIOPHYS CHEM,DEPT MOLEC BIOL,D-3400 GOTTINGEN,FED REP GER
来源
APPLIED OPTICS | 1979年 / 18卷 / 03期
关键词
D O I
10.1364/AO.18.000303
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
A unique light scattering photometer has been developed for continuous measurement of the complete angular spectrum of light scattered by dynamically changing systems. Although primarily designed for work with biological particles such as large viruses, subcellular organelles, and bacteria, it can be used to study polymerization and depolymerization of macromolecules, synthetic polymers, etc. The instrument has been applied to the direct measurement of the diameter of phospholipid vesicles and micelles in chromatographic column effluents and to the dynamics of polymerization of microtubular protein and osmotic lysis of chromaffin of granules. © 1979 Optical Society of America.
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页码:303 / 311
页数:9
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