BIOSYNTHESIS OF GLYCOLIPIDS . INCORPORATION OF N-ACETYL GALACTOSAMINE BY A RAT BRAIN PARTICULATE PREPARATION

被引：44
作者：
HANDA, S
BURTON, RM
机构：
[1] Department of Pharmacology and Beaumont-May Institute of Neurology, Washington University School of Medicine, St. Louis, 63110, Missouri
来源：
LIPIDS | 1969年 / 4卷 / 06期
关键词：
D O I：
10.1007/BF02531046
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A mixture of UDP-(N-acetyl)glucosamine-114C and UDP-(N-acetyl)galactosamine-114C (7:3) served as a substrate to demonstrate in young rat brain tissue the presence of enzymes which catalyzes the reactions: UDP-glcNAc⇌UDP-galNAc (E.C.5.1.3) and UDP-galNAc+ gal-glc-cer→ galNAc-gal-glc-cer+UDP. The glycolipid acceptor specificity was examined and preliminary kinetic data were obtained for the UDP-(N-acetyl)galactosamine: gal-glc-ceramide N-acetyl galactosamine transferase. The products of the reaction were identified. The relationship of this reaction to ganglioside biosynthesis is discussed. © 1969 The American Oil Chemists' Society.
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页码：589 / &
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