SOMACLONAL VARIATION IN RYE

被引:15
作者
LINACERO, R [1 ]
VAZQUEZ, AM [1 ]
机构
[1] UNIV COMPLUTENSE MADRID,FAC CIENCIAS BIOL,DEPT GENET,E-28040 MADRID,SPAIN
来源
MUTATION RESEARCH | 1993年 / 302卷 / 04期
关键词
SECALE; SOMACLONAL VARIATION; HIGH MUTABILITY; TRANSPOSABLE ELEMENTS;
D O I
10.1016/0165-7992(93)90105-5
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
We studied the genetic variation generated during in vitro culture of rye Secale cereale L. We analyzed the progenies of four generations of the plants regenerated from immature embryo cultures. A high frequency of mutant plants was observed, 50.75%, this frequency was genotype dependent. Other characteristics typical of somaclonal variation were also observed: the obtaining of dominant mutations, the presence of more than one mutation per plant, the obtaining of homozygous mutants and a high rate of mutation of particular loci. In some cases transposable elements could be implicated. We postulate that tissue culture could induce mutations as well as select particular cell types and so increase the appearance of special mutants.
引用
收藏
页码:201 / 205
页数:5
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