AUGMENTED PASSIVE TRANSFER OF CONTACT SENSITIVITY IN SEVERE COMBINED IMMUNODEFICIENCY MICE AND ITS DEPENDENCE OF V-BETA-8+ CELLS IN THE PICRYL SYSTEM

The passive transfer of contact sensitivity using picryl chloride immune cells from H-2 syngenic BALB/c donors was analyzed in severe combined immunodeficiency (SCID) mice which lack functional T and B lymphocytes. H-2-restricted and antigen-specific contact sensitivity was transferred to SCID mice, and comparison between the level of contact sensitivity and the number of transferred cells showed a significantly more efficient transfer to SCID than to BALB/c mice. The cells passively transferring contact sensitivity were shown to carry the Vbeta8 phenotype. Moreover, chromium-labeled cells from BALB/c PCl-primed donors localize normally in peripheral lymphoid organs, and an increased percentage of cell arrival in the ears is clearly observed in SCID after challenge with picryl chloride.