POTENTIAL TAXONOMIC USE OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) - A CASE-STUDY IN BRASSICA

被引：207
作者：
DEMEKE, T
ADAMS, RP
CHIBBAR, R
机构：
[1] BAYLOR UNIV, SANDY, UT 84093 USA
[2] NRC, INST PLANT BIOTECHNOL, SASKATOON S7N 0W9, SASKATCHEWAN, CANADA
来源：
THEORETICAL AND APPLIED GENETICS | 1992年 / 84卷 / 7-8期
关键词：
RAPDS; PCR; PRIMERS; BRASSICA; TAXONOMY;
D O I：
10.1007/BF00227415
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The potential use of RAPDs for taxonomic studies were investigated using Brassica, Sinapis and Raphanus taxa. Principal coordinate analysis of 284 RAPD bands revealed the classical U triangle relationship between diploid and amphidiploid Brassica taxa. Raphanus sativus and S. alba were distinct from the Brassica taxa. It appears that at least ten primers with approximately 100 total bands are needed to adequately portray these relationships. Cultivars of cabbage and cauliflower were separated by RAPDs. Analysis of RAPDs from individual plants of B. carinata cv. dodola resulted in 69 RAPDs, with 91.7% monomorphic and 8.3% polymorphic bands. RAPDs appear to be useful for taxonomic studies at levels ranging from populations to species and perhaps genera.
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页码：990 / 994
页数：5
相关论文
共 22 条
[1]   STATISTICAL CHARACTER WEIGHTING AND SIMILARITY STABILITY [J].
ADAMS, RP .
BRITTONIA, 1975, 27 (04) :305-316
[2]   DNA AMPLIFICATION FINGERPRINTING USING VERY SHORT ARBITRARY OLIGONUCLEOTIDE PRIMERS [J].
CAETANOANOLLES, G ;
BASSAM, BJ ;
GRESSHOFF, PM .
BIO-TECHNOLOGY, 1991, 9 (06) :553-557
[3]   SEGREGATION OF RANDOM AMPLIFIED DNA MARKERS IN F1 PROGENY OF CONIFERS [J].
CARLSON, JE ;
TULSIERAM, LK ;
GLAUBITZ, JC ;
LUK, VWK ;
KAUFFELDT, C ;
RUTLEDGE, R .
THEORETICAL AND APPLIED GENETICS, 1991, 83 (02) :194-200
[4]  
Doyle J. J., 1987, PHYTOCHEMISTRY B, V19, P11, DOI DOI 10.2307/4119796
[5]  
GOWER JC, 1966, BIOMETRIKA, V53, P325, DOI 10.2307/2333639
[6]   GENERAL COEFFICIENT OF SIMILARITY AND SOME OF ITS PROPERTIES [J].
GOWER, JC .
BIOMETRICS, 1971, 27 (04) :857-&
[7]   DEVELOPMENT AND CHROMOSOMAL LOCALIZATION OF GENOME-SPECIFIC DNA MARKERS OF BRASSICA AND THE EVOLUTION OF AMPHIDIPLOIDS AND N = 9 DIPLOID SPECIES [J].
HOSAKA, K ;
KIANIAN, SF ;
MCGRATH, JM ;
QUIROS, CF .
GENOME, 1990, 33 (01) :131-142
[8]  
HU J, 1991, PLANT CELL REP, V10, P505, DOI 10.1007/BF00234583
[9]   ISOLATION OF MOLECULAR MARKERS FOR TOMATO (LYCOPERSICON-ESCULENTUM) USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) [J].
KLEINLANKHORST, RM ;
VERMUNT, A ;
WEIDE, R ;
LIHARSKA, T ;
ZABEL, P .
THEORETICAL AND APPLIED GENETICS, 1991, 83 (01) :108-114
[10]   RAPID IDENTIFICATION OF MARKERS LINKED TO A PSEUDOMONAS RESISTANCE GENE IN TOMATO BY USING RANDOM PRIMERS AND NEAR-ISOGENIC LINES [J].
MARTIN, GB ;
WILLIAMS, JGK ;
TANKSLEY, SD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2336-2340
123