DETERMINATION OF CLOZAPINE AND DESMETHYLCLOZAPINE IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

被引：56
作者：
CHUNG, MC
LIN, SK
CHANG, WH
JANN, MW
机构：
[1] TAIPEI CITY PSYCHIAT CTR,TAIPEI,TAIWAN
[2] MERCER UNIV,SO SCH PHARM,ATLANTA,GA 30341
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 613卷 / 01期
关键词：
D O I：
10.1016/0378-4347(93)80212-M
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method using reversed-phase high-performance liquid chromatography for the simultaneous determination of clozapine and its desmethyl metabolite in human plasma has been established. Clozapine and N-desmethylclozapine were extracted with n-hexane-isoamyl aclohol (98.5:1.5, v/v). Protriptyline served as the internal standard. The limits of detection for clozapine and desmethylclozapine are 2 and 1 ng/ml, respectively. The sensitivity and precision of this method can be utilized for pharmacokinetic studies and therapeutic drug monitoring regimens.
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收藏
页码：168 / 173
页数：6
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