SUBUNITS OF THE EXTRACELLULAR HEMOGLOBIN OF TUBIFEX-TUBIFEX

被引：21
作者：
VINOGRADOV, SN
HERSEY, SL
FROHMAN, C
KAPP, OH
机构：
[1] Biochemistry Department, Wayne State University School of Medicine, Detroit
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 578卷 / 01期
关键词：
(Annelid); Equilibrium sedimentations; Hemoglobin; Molecular weight;
D O I：
10.1016/0005-2795(79)90129-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The molecular weight of the extracellular hemoglobin of Tubifex tubifex determined by equilibrium sedimentation is 3.0 ± 0.2 · 106. Polyacrylamide gel electrophoresis in sodium dodecyl sulfate showed that the hemoglobin dissociated into four subunits: 13 000 (subunit 1), 21 000 (subunit 2), 23 000 (subunit 3) and 47 000 (subunit 4); in the presence of mercaptoethanol two subunits were observed, 13 000 ± 1000 (subunit I) accounting for 70-80% of the whole molecule, and 26 000 (subunit II). Electrophoresis of the subunits obtained in the absence of mercaptoethanol showed that subunit I originated from subunits 1 and 4, while subunit II originated from subunits 2 and 3. These relationships were supported by N-terminal group determinations. Gel filtration in 6 M guanidine hydrochloride showed that the molecular weight of subunit I is 17 500 and that of subunit II, 36 000. Tubifex hemoglobin appears to consist of at least seven polypeptide chains. © 1979.
引用
收藏
页码：216 / 222
页数：7
相关论文
共 50 条
[21]   Renal Toxicodynamic Effects of Extracellular Hemoglobin After Acute Exposure [J].
Baek, Jin Hyen ;
Yalamanoglu, Ayla ;
Brown, Ronald P. ;
Saylor, David M. ;
Malinauskas, Richard A. ;
Buehler, Paul W. .
TOXICOLOGICAL SCIENCES, 2018, 166 (01) :180-191
[22]   Molecular mass of macromolecules and subunits and the quaternary structure of hemoglobin from the microcrustacean Daphnia magna [J].
Lamkemeyer, Tobias ;
Zeis, Bettina ;
Decker, Heinz ;
Jaenicke, Elmar ;
Waschbuesch, Dieter ;
Gebauer, Wolfgang ;
Markl, Juergen ;
Meissner, Ulrich ;
Rousselot, Morgane ;
Zal, Franck ;
Nicholson, Graeme J. ;
Paul, Ruediger J. .
FEBS JOURNAL, 2006, 273 (14) :3393-3410
[23]   Clinical Application of Haptoglobin in Models of Supraphysiologic Extracellular Brain Hemoglobin Levels [J].
Leclerc, Jenna L. ;
Robbins, Sean ;
Esfandiary, Tina ;
Dang, Alex ;
Dore, Sylvain .
STROKE, 2015, 46
[24]   GIANT EXTRACELLULAR HEMOGLOBIN OF Glossoscolex paulistus: AN EXTRAORDINARY SUPRAMOLECULAR HEMOPROTEIN SYSTEM [J].
Moreira, Leonardo Marmo ;
Guaranho de Moraes, Pedro Claudio ;
Rodrigues Furtado de Mendonca, Jose Paulo ;
Guimaraes, Luciana ;
Lyon, Juliana Pereira ;
Aimbire, Flavio ;
Poli, Alessandra Lima ;
Imasato, Hidetake .
QUIMICA NOVA, 2011, 34 (01) :119-130
[25]   The Role of Extracellular Ca2+ in Regulating the Distribution and Conformation of Hemoglobin in Erythrocytes [J].
O. V. Slatinskaya ;
N. A. Brazhe ;
S. N. Orlov ;
G. V. Maksimov .
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology, 2021, 15 :230-238
[26]   The Role of Extracellular Ca2+ in Regulating the Distribution and Conformation of Hemoglobin in Erythrocytes [J].
Slatinskaya, O., V ;
Brazhe, N. A. ;
Orlov, S. N. ;
Maksimov, G., V .
BIOLOGICHESKIE MEMBRANY, 2021, 38 (03) :199-208
[27]   Aortic Valve Stenosis Causes Accumulation of Extracellular Hemoglobin and Systemic Endothelial Dysfunction [J].
Quast, Christine ;
Boenner, Florian ;
Polzin, Amin ;
Veulemans, Verena ;
Chennupati, Ramesh ;
Gyamfi Poku, Isabella ;
Pfeiler, Susanne ;
Kramser, Nicolas ;
Nankinova, Magdalena ;
Staub, Nicole ;
Zweck, Elric ;
Jokiel, Juliane ;
Keyser, Fabian ;
Hoffe, Jasmina ;
Witkowski, Sven ;
Becker, Katrin ;
Leuders, Pia ;
Zako, Saif ;
Erkens, Ralf ;
Jung, Christian ;
Floegel, Ulrich ;
Wang, Tianai ;
Neidlin, Michael ;
Steinseifer, Ulrich ;
Niepmann, Sven Thomas ;
Zimmer, Sebastian ;
Gerdes, Norbert ;
Cortese-Krott, Miriam M. ;
Feelisch, Martin ;
Zeus, Tobias ;
Kelm, Malte .
CIRCULATION, 2024, 150 (12) :952-965
[28]   Expression of Vitreoscilla hemoglobin improves growth and levels of extracellular enzyme in Yarrowia lipolytica [J].
Bhave, SL ;
Chattoo, BB .
BIOTECHNOLOGY AND BIOENGINEERING, 2003, 84 (06) :658-666
[29]   The Role of Extracellular Ca2+ in Regulating the Distribution and Conformation of Hemoglobin in Erythrocytes [J].
Slatinskaya, O., V ;
Brazhe, N. A. ;
Orlov, S. N. ;
Maksimov, G., V .
BIOCHEMISTRY MOSCOW SUPPLEMENT SERIES A-MEMBRANE AND CELL BIOLOGY, 2021, 15 (03) :230-238
[30]   Role of Haptoglobin in Protection from the Toxic Effects of Extracellular Hemoglobin in Various Pathologies [J].
M. G. Golubeva .
Biology Bulletin Reviews, 2023, 13 (4) :293-300
12345