Reduced levels of class 1 reversibly glycosylated polypeptide increase intercellular transport via plasmodesmata

被引：18
作者：
Burch-Smith, Tessa M. [1 ]
Cui, Ya [1 ]
Zambryski, Patricia C. [1 ]
机构：
[1] Univ Calif Berkeley, Dept Plant & Microbial Biol, Koshland Hall, Berkeley, CA 94720 USA
来源：
PLANT SIGNALING & BEHAVIOR | 2012年 / 7卷 / 01期
关键词：
plasmodesmata; reversibly glycosylated polypeptide; virus-induced gene silencing; tobacco mosaic virus; TMV movement protein;
D O I：
10.4161/psb.7.1.18636
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Maize and Arabidopsis thaliana class 1 reversibly glycosylated polypeptides (C1RGPs) are plasmodesmata-associated proteins. Previously, overexpression of Arabidopsis (C1)RGP AtRGP2 in Nicotiana tabacum was shown to reduce intercellular transport of photoassimilate, resulting in stunted, chlorotic plants, and inhibition of local cell-to-cell spread of tobacco mosaic virus (TMV). Here, we used virus induced gene silencing to examine the effects of reduced levels of (C1)RGPs in Nicotiana benthamiana. Silenced plants show wild-type growth and development. Intercellular transport in silenced plants was probed using fluorescently labeled TMV and its movement protein, P30. P30 shows increased cell-to-cell movement and TMV exhibited accelerated systemic spread compared with control plants. These results support the hypothesis that (C1)RGPs act to regulate intercellular transport via plasmodesmata.
引用
收藏
页码：62 / 67
页数：6
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