BENZO[A]PYRENE-DIONE-STIMULATED OXYRADICAL PRODUCTION BY MICROSOMES OF DIGESTIVE GLAND OF THE COMMON MUSSEL, MYTILUS-EDULIS-L

The potential of benzo[a]pyrene (BaP) quinones (diones) to stimulate NAD(P)H-dependent hydroxyl radical (. OH) production (2 -keto-4-methiolbutyric acid (KMBA) oxidation) by digestive gland microsomes of M. edulis was studied using iron/EDTA as a promotor of the Haber-Weiss reaction (O-2(-) + H2O2 = . OH + OH- + O-2). Stimulation of basal rates of KMBA oxidation was observed for all three diones. Stimulation was similar for the 1,6- and 3,6-diones, but much less for the 6,12-dione, and greater for the NADH than the NADPH-dependent reaction, viz. % increases of 78 to 333 (NADH) compared to 0 to 78 (NADPH). Maximal KMBA oxidation was obtained at dione concentrations of 12.5 to 50 mu M. Inhibition of KMBA oxidation by superoxide dismutase and catalase indicated the involvement of respectively superoxide anion radical (O-2(-)) and hydrogen peroxide (H2O2) in 1,6-dione stimulated NADPH-dependent . OH formation. The apparent K-m values of xenobiotic-stimulated KMBA oxidation were lower for BaP-diones than for the model redox cycling quinone menadione (2-methyl-1,4-naphthoquinone), viz. in mu M, 0.6 to 14.6 (NADH) and 4.4 to 28.5 (NADPH) compared to 315 to 457 (NADH) and 72 to 103 (NADPH). The results are consistent with metabolism of BaP to diones and resultant enhanced generation of oxyradicals being a potential mechanism of pollutant-mediated toxicity in molluscs.