DESIGN AND USE OF SYNTHETIC OLIGONUCLEOTIDE PROBES IN THE CLONING OF DELTA-ENDOTOXIN GENES FROM BACILLUS-THURINGIENSIS

被引：0

作者：

STARKEY, MP

FENNING, T

DAVEY, MR

MULLIGAN, BJ

机构：

[1] UNIV NOTTINGHAM,DEPT BOT,NOTTINGHAM NG7 2RD,ENGLAND

[2] HATFIELD POLYTECH,SCH NAT SCI,HATFIELD AL10 9AB,HERTS,ENGLAND

[3] HRI,MAIDSTONE,KENT,ENGLAND

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1991年 / 13卷 / 08期

关键词：

BACILLUS-THURINGIENSIS; CRYSTAL TOXIN; OLIGONUCLEOTIDE PROBES; HYBRIDIZATION METHODOLOGY; GENE CLONING;

D O I：

10.1016/0141-0229(91)90081-K

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A detailed protocol is described for the design and use of synthetic oligonucleotide probes for screening DNA libraries from Bacillus thuringiensis var. kurstaki (strain HD191) for copies of the gene (tox) encoding the insecticidal delta-endotoxin. Two homologous tox genes were identified in this organism; one of these was located on a 75-kb plasmid and the other on a second large plasmid or the bacterial chromosome. A tox gene was isolated as a 6.5-kb HindIII fragment of B. thuringiensis plasmid DNA.

引用

页码：661 / 664

页数：4

共 37 条

[1] CHARACTERIZED FULL-LENGTH AND TRUNCATED PLASMID CLONES OF THE CRYSTAL PROTEIN OF BACILLUS-THURINGIENSIS SUBSP KURSTAKI HD-73 AND THEIR TOXICITY TO MANDUCA-SEXTA [J].