REPAIR OF GAMMA RAY-INDUCED S1 NUCLEASE HYPERSENSITIVE SITES IN YEAST DEPENDS ON HOMOLOGOUS MITOTIC RECOMBINATION AND A RAD18-DEPENDENT FUNCTION

被引：23

作者：

GEIGL, EM ^{[1
]}

ECKARDTSCHUPP, F ^{[1
]}

机构：

[1] GESELL STRAHLEN & UMWELTFORSCH MBH,FORSCHUNGSZENTRUM UMWELT & GESUNDHEIT,INST STRAHLENBIOL,W-8042 NEUHERBERG,GERMANY

来源：

CURRENT GENETICS | 1991年 / 20卷 / 1-2期

关键词：

PULSED FIELD GEL-ELECTROPHORESIS; S1 NUCLEASE SENSITIVE SITES; REPAIR; YEAST;

D O I：

10.1007/BF00312762

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Repair under non-growth conditions of DNA double-strand breaks (DSB) and chromatin sites sensitive to S1 endonuclease (SSS) induced by Cobalt-60-gamma rays were monitored in repair-competent and deficient strains of Saccharomyces cerevisiae by pulsed field gel-electrophoresis. In stationary-phase cells of a repair-competent RAD diploid, and an excision-deficient rad3-2 diploid, SSS are repaired as efficiently as DSB, whereas in a repair-competent RAD haploid, and a rad 50-1 diploid, neither SSS nor DSB are repaired. The rad18-2 diploid repairs DSB well but is defective in SSS repair. Obviously, SSS repair in yeast chromatin, like DSB repair, depends on recombination, but unlike DSB repair depends additionally on RAD18 function.

引用

页码：33 / 37

页数：5

共 45 条

[31] EXPRESSION OF THE SACCHAROMYCES-CEREVISIAE DNA-REPAIR GENE RAD6 THAT ENCODES A UBIQUITIN CONJUGATING ENZYME, INCREASES IN RESPONSE TO DNA DAMAGE AND IN MEIOSIS BUT REMAINS CONSTANT DURING THE MITOTIC CELL-CYCLE [J].