LEVELS OF GLUTATHIONE, GLUTATHIONE REDUCTASE AND GLUTATHIONE S-TRANSFERASE ACTIVITIES IN RAT LUNG AND LIVER

被引：3221

作者：

MORON, MS

DEPIERRE, JW

MANNERVIK, B

机构：

[1] Department of Biochemistry, Arrhenius Laboratory, University of Stockholm

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 582卷 / 01期

基金：

美国国家卫生研究院;

关键词：

(Rat lung); Glutathione; Glutathione reductase; Glutathione S-transferase;

D O I：

10.1016/0304-4165(79)90289-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver have been investigated. After perfusing the lung to remove contaminating blood, this organ was found to have an apparent concentration of glutathione (2 mM) which is approx. 20% of that found in the liver. Both organs contain very low levels of glutathione disulfide. Neither phenobarbital nor methylcholanthrene had a significant effect on the levels of reduced glutathione in the lung and liver. In addition, the activities of some glutathine-metabolizing enzymes - glutathione reductase and glutathione S-transferase activity assayed with four different substances - were observed to be 5- to 60-fold lower in lung tissue than in the liver. © 1979.

引用

页码：67 / 78

页数：12

共 38 条

[1]

Arias I. M., 1976, GLUTATHIONE METABOLI, P175

[2] MECHANISM OF ACTION OF ENZYMES CATALYZING THIOL-DISULFIDE INTERCHANGE - THIOLTRANSFERASES RATHER THAN TRANSHYDROGENASES [J].