ISOLATION AND CHARACTERIZATION OF ADDITION-PRODUCTS OF ALPHA-TOCOPHEROL WITH PEROXYL RADICALS OF DILINOLEOYLPHOSPHATIDYLCHOLINE IN LIPOSOMES

被引：27

作者：

YAMAUCHI, R

YAGI, Y

KATO, K

机构：

[1] Department of Food Science, Faculty of Agriculture, Gifu University, Gifu City, Gifu, 501-11

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1994年 / 1212卷 / 01期

关键词：

ALPHA-TOCOPHEROL; PEROXYL RADICAL; LIPID PEROXIDATION; LIPOSOME; PHOSPHATIDYLCHOLINE;

D O I：

10.1016/0005-2760(94)90187-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha-Tocopherol was reacted with peroxyl radicals of phosphatidylcholine at 37 degrees C in liposomes. The phospholipid-peroxyl radicals were generated by the reaction of 1,2-dilinoleoyl-3-sn-phosphatidylcholine with a free radical initiator, 2,2'-azobis(2,4-dimethylvaleronitrile), under air. One peak corresponding to the reaction products of alpha-tocopherol with phosphatidylcholine-peroxyl radicals was isolated by reversed-phase high-performance liquid chromatography. Its structure was identified as a mixture of 8a-(phosphatidylcholine-peroxy)-alpha-tocopherones: 1-[9-(8a-peroxy-alpha-tocopherone)-10,12-octadecadienoyl]-2-linoleoyl-3-sn-phosphatidylcholine,1-[13-(8a-peroxy-alpha-tocopherone)-9,11-octadecadienoyl]-2-linoleoyl-3-sn-phosphatidylcholine, 1-linoleoyl-2-[9-(8a-peroxy-alpha-tocopherone)-10,12-octadecadienoyl]-3-sn-phosphatidylcholine and 1-linoleoyl-2-[13-(8a-peroxy-alpha-tocopherone)-9,11-octadecadienoyl]-3-sn-phosphatidylcholine. The results indicate that each alpha-tocopherol can trap two peroxyl radicals during the peroxidation of unsaturated phospholipid in liposomes.

引用

页码：43 / 49

页数：7

共 22 条

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