Capsicum Annum linn. is locally known as dry chili in Bangladesh. It is used as spices all over the world. In this study fatty oil of the two varieties of dry chilly from two different places of Bangladesh were analyzed by GC-MS. Dry chili (Capsicum Annum linn.) Fatty oil has been widely used1 industrially as an ingredient of many products, e.g. hot sauces, canned fish, ginger beer, as well as for some pharmaceutical products. Fatty oil of two varieties of dry chili was analysis by GC-MS and their composition were established. The GC-MS analysis showed that, Dinajpur's dry chili contained total nine fatty oils. These were Tetradecanoic acid, methyl ester (2.72%); 9-Hexadecanoic acid, methyl ester (1.55%); Hexadecanoic acid, methyl ester (17.40%); 9,12-Octadecadienoic acid(Z,Z)-methyl ester (44.64%); 10-Octadecanoic acid, methyl ester (24.33%); 9-octadecenoic acid(z)-methyl ester (2.39%); Octadecanoic acid, methyl ester (3.76%); Octadecanoic acid, 9,10,12-trimethoxy-, methyl ester (1.43%); 4-tert-butyl-2-methoxyquinoline-3-phenyl-2-piperidino-2-propen-1-a 4-butyl-2,2-dimethyl-2H-1-benzopyran (1.80%). On the other hand, Chittagong's dry chili contained total ten fatty oils. These were Dodecanoic acid, methyl ester (1.99%); Tetradecanoic acid, methyl ester(5.69%); 9-Hexadecanoic acid, methyl ester (1.14%); Hexadecanoic acid, methyl ester (30.15%); 9,12-Octadecadienoic acid(Z, Z)-methyl ester (29.30%); 9-octadecenoic acid(z)-methyl ester(17.82%); 8-octadecenoic acid, methyl ester(3.08%); Octadecanoic acid, methyl ester (5.48%); Octadecanoic acid, 9,10,12-trimethoxy-, methyl ester (2.02%); Methoxylate ester of methyl oleate (3.34%). Elemental analysis was also performed by ED-XRF Analyzer and was observed the mineral contents in fatty oil of the samples. In the minerals, % of K was major in both the samples (85.16% for Dinajpur and 82.54% for Chittagong).