BRADYKININ AND VASOPRESSIN ACTIVATE PHOSPHOLIPASE-D IN RAT LEYDIG-CELLS BY A PROTEIN KINASE-C-DEPENDENT MECHANISM

被引:15
作者
VINGGAARD, AM
HANSEN, HS
机构
[1] PharmaBiotec Research Centre, Department of Biological Sciences, Royal Danish School of Pharmacy, DK-2100 Copenhagen
关键词
D O I
10.1677/joe.0.1360119
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In the present study we report that bradykinin stimulated phospholipase D activity in rat Leydig cells. Bradykinin added for 8 min stimulated choline formation in a dose-dependent manner and, in the presence of ethanol, bradykinin (100 nmol/l) stimulated transphosphatidylation by phospholipase D resulting in the formation of phosphatidylethanol. This stimulation was abolished after down-regulation of protein kinase C by long-term pretreatment for 22 h with phorbol 12-myristate 13-acetate (PMA). The stimulation of phospholipase D by the simultaneous addition for 8 min of maximum concentrations of PMA and vasopressin (AVP), PMA and bradykinin, or AVP and bradykinin produced no additive phosphatidylethanol or choline response, suggesting that AVP, bradykinin and PMA stimulated phospholipase D-catalysed phosphatidylcholine hydrolysis by a similar protein kinase C-dependent mechanism. Furthermore, LH (10 ng/ml), insulin (500 nmol/l, GH (100 ng/ml), interleukin-1beta (5 U/ml) and platelet-activating factor (200 nmol/l) were found not to activate phospholipase D, whereas the Ca2+ ionophore A23187 (10 mumol/l) stimulated phosphatidylethanol formation, suggesting that Ca2+ might be a regulator of phospholipase D in Leydig cells.
引用
收藏
页码:119 / 126
页数:8
相关论文
共 35 条
[1]   EVIDENCE FOR THE INVOLVEMENT OF PHOSPHOLIPASE-A2 IN THE REGULATION OF LUTEINIZING HORMONE-STIMULATED STEROIDOGENESIS IN RAT TESTIS LEYDIG-CELLS [J].
ABAYASEKARA, DRE ;
BAND, AM ;
COOKE, BA .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1990, 70 (02) :147-153
[2]   DIRECT INHIBITION OF TESTICULAR ANDROGEN BIOSYNTHESIS REVEALING ANTI-GONADAL ACTIVITY OF NEUROHYPOPHYSEAL HORMONES [J].
ADASHI, EY ;
HSUEH, AJW .
NATURE, 1981, 293 (5834) :650-652
[3]  
ADASHI EY, 1984, J BIOL CHEM, V259, P5440
[4]   THE REGULATION AND CELLULAR FUNCTIONS OF PHOSPHATIDYLCHOLINE HYDROLYSIS [J].
BILLAH, MM ;
ANTHES, JC .
BIOCHEMICAL JOURNAL, 1990, 269 (02) :281-291
[5]   PHOSPHATIDATE-DEPENDENT PROTEIN-PHOSPHORYLATION [J].
BOCCKINO, SB ;
WILSON, PB ;
EXTON, JH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (14) :6210-6213
[6]   EFFECT OF DIFFERENT STEROIDOGENIC STIMULI ON PROTEIN-PHOSPHORYLATION AND STEROIDOGENESIS IN MA-10 MOUSE LEYDIG TUMOR-CELLS [J].
CHAUDHARY, LR ;
STOCCO, DM .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1094 (02) :175-184
[7]   IS CYCLIC-AMP AN OBLIGATORY 2ND MESSENGER FOR LUTEINIZING-HORMONE [J].
COOKE, BA .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1990, 69 (01) :C11-C15
[9]   INVITRO MODULATION OF PORCINE LEYDIG-CELL STEROIDOGENESIS BY PHORBOL-12-MYRISTATE-13-ACETATE AND 1,2-DIOCTANOYLGLYCEROL [J].
FRENCH, JT ;
WELSH, TH .
ACTA ENDOCRINOLOGICA, 1990, 122 (01) :101-106
[10]   PHOSPHATIDYLETHANOL FORMATION IN RAT HEPATOCYTES [J].
GUSTAVSSON, L ;
MOEHREN, G ;
HOEK, JB .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1991, 625 :438-440