OPTIMIZING MOBILE-PHASE COMPOSITION, ITS FLOW-RATE AND COLUMN TEMPERATURE IN HPLC USING AN EXPERIMENTAL-DESIGN ASSISTED WITH A SIMPLEX-METHOD

被引：16

作者：

GUILLAUME, Y

GUINCHARD, C

机构：

[1] Laboratoire de Chimie Analytique, UFR des Sciences Médicales et Pharmaceutiques, Besancon, Place St. Jacques

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1993年 / 16卷 / 16期

关键词：

D O I：

10.1080/10826079308019701

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work, a rapid procedure to separate ten compounds in high performance liquid chromatography is presented. The use of an experimental design assisted with a simplex method is proposed to separate these compounds with only thirteen chromatographic analyses to select the mobile phase composition, its flow rate and the column temperature. A flow rate of 0.77 ml/min with a percentage of methanol of 49,95% in the mixture methanol-water and a column temperature of 51.62-degrees-C gave the most efficient separation conditions.

引用

页码：3457 / 3470

页数：14

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