MOLECULAR-CLONING AND CHROMOSOMAL MAPPING OF CCND GENES ENCODING HUMAN D-TYPE CYCLINS

被引：213

作者：

XIONG, Y ^{[1
]}

MENNINGER, J ^{[1
]}

BEACH, D ^{[1
]}

WARD, DC ^{[1
]}

机构：

[1] YALE UNIV, SCH MED, DEPT GENET, NEW HAVEN, CT 06510 USA

来源：

GENOMICS | 1992年 / 13卷 / 03期

关键词：

D O I：

10.1016/0888-7543(92)90127-E

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A human D-type cyclin gene (CCND1/cyclin D1 PRAD1) was previously isolated by virtue of its ability to complement a triple G1 cyclin (Cln) deficiency of Saccharomyces cerevisiae and was also identified as a candidate BCL1 oncogene. We now report the molecular cloning of two additional human D-type cyclin genes, CCND2 (cyclin D2) and CCND3 (cyclin D3). Allthree human D-type cyclin genes encode small (33-34 kDa) proteins that share an average of 57% identity over the entire coding region and 78% in the cyclin box. The D-type cyclins are most closely related to cyclin A (39% identity) and cyclin E (36%), followed by cyclin B (29%) and cyclin C (21%). Isolation and characterization of genomic clones revealed two pseudogenes corresponding to CCND2 and CCND3, respectively. All three cyclin D genes are interrupted by an intron at the same position. CCND2 has been mapped to chromosome 12p13, and CCND3 has been mapped to chromosome 6p21. © 1992.

引用

页码：575 / 584

页数：10