MOLECULAR-CLONING AND CHROMOSOMAL MAPPING OF CCND GENES ENCODING HUMAN D-TYPE CYCLINS

被引：213

作者：

XIONG, Y ^{[1
]}

MENNINGER, J ^{[1
]}

BEACH, D ^{[1
]}

WARD, DC ^{[1
]}

机构：

[1] YALE UNIV, SCH MED, DEPT GENET, NEW HAVEN, CT 06510 USA

来源：

GENOMICS | 1992年 / 13卷 / 03期

关键词：

D O I：

10.1016/0888-7543(92)90127-E

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A human D-type cyclin gene (CCND1/cyclin D1 PRAD1) was previously isolated by virtue of its ability to complement a triple G1 cyclin (Cln) deficiency of Saccharomyces cerevisiae and was also identified as a candidate BCL1 oncogene. We now report the molecular cloning of two additional human D-type cyclin genes, CCND2 (cyclin D2) and CCND3 (cyclin D3). Allthree human D-type cyclin genes encode small (33-34 kDa) proteins that share an average of 57% identity over the entire coding region and 78% in the cyclin box. The D-type cyclins are most closely related to cyclin A (39% identity) and cyclin E (36%), followed by cyclin B (29%) and cyclin C (21%). Isolation and characterization of genomic clones revealed two pseudogenes corresponding to CCND2 and CCND3, respectively. All three cyclin D genes are interrupted by an intron at the same position. CCND2 has been mapped to chromosome 12p13, and CCND3 has been mapped to chromosome 6p21. © 1992.

引用

页码：575 / 584

页数：10

共 33 条

[1] INSITU HYBRIDIZATION BANDING OF HUMAN-CHROMOSOMES WITH ALU-PCR PRODUCTS - A SIMULTANEOUS KARYOTYPE FOR GENE-MAPPING STUDIES [J].

BALDINI, A ;

WARD, DC .

GENOMICS, 1991, 9 (04) :770-774

[2]

BOYLE AL, 1991, CURRENT PROTOCOLS MO

[3] DETECTION OF VIRAL GENOMES IN CULTURED-CELLS AND PARAFFIN-EMBEDDED TISSUE-SECTIONS USING BIOTIN-LABELED HYBRIDIZATION PROBES [J].

BRIGATI, DJ ;

MYERSON, D ;

LEARY, JJ ;

SPALHOLZ, B ;

TRAVIS, SZ ;

FONG, CKY ;

HSIUNG, GD ;

WARD, DC .

VIROLOGY, 1983, 126 (01) :32-50

[4] PATHOGENESIS OF ADULT TESTICULAR GERM-CELL TUMORS - A CYTOGENETIC MODEL [J].

DEJONG, B ;

OOSTERHUIS, JW ;

CASTEDO, SMMJ ;

VOS, A ;

TEMEERMAN, GJ .

CANCER GENETICS AND CYTOGENETICS, 1990, 48 (02) :143-167

[5] CDC2 PROTEIN-KINASE IS COMPLEXED WITH BOTH CYCLIN-A AND CYCLIN-B - EVIDENCE FOR PROTEOLYTIC INACTIVATION OF MPF [J].

DRAETTA, G ;

LUCA, F ;

WESTENDORF, J ;

BRIZUELA, L ;

RUDERMAN, J ;

BEACH, D .

CELL, 1989, 56 (05) :829-838

[6] IDENTIFICATION OF 2 HUMAN FERRITIN-H GENES ON THE SHORT ARM OF CHROMOSOME-6 [J].

DUGAST, IJ ;

PAPADOPOULOS, P ;

ZAPPONE, E ;

JONES, C ;

THERIAULT, K ;

HANDELMAN, GJ ;

BENAROUS, R ;

DRYSDALE, JW .

GENOMICS, 1990, 6 (02) :204-211

[7] CYCLIN - A PROTEIN SPECIFIED BY MATERNAL MESSENGER-RNA IN SEA-URCHIN EGGS THAT IS DESTROYED AT EACH CLEAVAGE DIVISION [J].

EVANS, T ;

ROSENTHAL, ET ;

YOUNGBLOM, J ;

DISTEL, D ;

HUNT, T .

CELL, 1983, 33 (02) :389-396

[8] EVIDENCE THAT THE G1-S AND G2-M TRANSITIONS ARE CONTROLLED BY DIFFERENT CDC2 PROTEINS IN HIGHER EUKARYOTES [J].

FANG, F ;

NEWPORT, JW .

CELL, 1991, 66 (04) :731-742

[9] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[10] A 60 KD CDC2-ASSOCIATED POLYPEPTIDE COMPLEXES WITH THE E1A PROTEINS IN ADENOVIRUS-INFECTED CELLS [J].

GIORDANO, A ;

WHYTE, P ;

HARLOW, E ;

FRANZA, BR ;

BEACH, D ;

DRAETTA, G .

CELL, 1989, 58 (05) :981-990

← 1 2 3 4 →