Accelerated Healing of Ulcer Wounds in the Rabbit Ear by Recombinant Human Transforming Growth Factor-beta 1

被引：36

作者：

Beck, L. Steven ^{[1
]}

Chen, Theresa L. ^{[1
]}

Hirabayashi, Sue E. ^{[1
]}

Deguzman, Leo ^{[1
]}

Lee, Wyne P. ^{[1
]}

McFatridge, Lorrie L. ^{[1
]}

Xu, Yvette ^{[1
]}

Bates, Rebecca L. ^{[1
]}

Ammann, Arthur J. ^{[1
]}

机构：

[1] Genentech Inc, Dept Pharmacol Sci, 460 Point San Bruno Blvd, San Francisco, CA 94080 USA

来源：

GROWTH FACTORS | 1990年 / 2卷 / 03期

关键词：

recombinant human transforming growth factor-beta 1; wound healing ulcers;

D O I：

10.3109/08977199009078015

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A dermal ulcer wound-healing model was established in rabbit ear to examine the effects of recombinant human transforming growth factor-beta 1 (rhTGF-beta 1) in wound healing. Histomorphometric examination of the wounds indicate a biphasic healing response 7 days after a single application of rhTGF beta 1 at the time of wounding. Statistically significant healing occurred at 5-100 ng but not at higher doses of 300 or 1000 ng rhTGF-beta 1/wound. Enhanced collagen synthesis as determined by [H-3]proline incorporation occurred at 15 and 25 ng and was significantly depressed at 500 ng rhTGF-beta 1/wound. Multiple doses of 100 ng rhTGF-gl applied to the wound at the time of wounding and for 3 days after wounding provided results comparable to the single application of growth factor. Delaying treatment 24 hr after wounding did not enhance wound healing compared with vehicle. Our findings suggest that rhTGF-g1 can be a valuable growth factor to improve the healing of ulcer wounds.

引用

页码：273 / 282

页数：10

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