ANALYSIS OF NIFEDIPINE AND ITS PYRIDINE METABOLITE DEHYDRONIFEDIPINE IN BLOOD AND PLASMA - REVIEW AND IMPROVED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHODOLOGY

A reversed-phase HPLC method is described for the simultaneous determination of nifedipine and its primary pyridine metabolite dehydronifedipine in blood and plasma, that involves UV detection and neutral (blood) or alkaline (plasma) extraction. The limit of reliable determination is found to be 3 ng ml-1 with an inter-assay RSD of below 11%. In the presence of haemoglobin, nifedipine is unstable at pH > 10, necessitating neutral extraction for the measurement of nifedipine in haemolysed blood. Published methods for analysis of nifedipine are reviewed, emphasizing the lack of specificity and sensitivity which render many of them unsuitable for the investigation of nifedipine disposition in man.