IDENTIFICATION OF 11-CIS AND ALL-TRANS-RETINAL IN THE PHOTORECEPTIVE ORGANELLE OF A FLAGELLATE GREEN-ALGA

被引：35
作者：
KREIMER, G [1 ]
MARNER, FJ [1 ]
BROHSONN, U [1 ]
MELKONIAN, M [1 ]
机构：
[1] UNIV COLOGNE,INST BIOCHEM,W-5000 COLOGNE 41,GERMANY
来源：
FEBS LETTERS | 1991年 / 293卷 / 1-2期
关键词：
RETINAL; PHOTOTAXIS; PHOTORECEPTIVE ORGANELLE; EYESPOT; GREEN ALGAE;
D O I：
10.1016/0014-5793(91)81150-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Isolation of intact photoreceptive organelles (eyespot apparatuses) involved in blue-light mediated photoresponses in a flagellate green alga (Spermatozopsis similis) allowed for the first time the identification of both 11-cis- and all-trans-retinal in a plant cell. Both isomers were identified by HPLC analysis in conjunction with UV spectra. Additionally, reconstitution of a distinct absorption band, centered around 540 nm, was achieved by addition of exogenous 9-cis-retinal to bleached, isolated eyespot apparatuses.
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收藏
页码：49 / 52
页数：4
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