A COMBINED PHYSICAL AND GENETIC-MAP OF PSEUDOMONAS-AERUGINOSA PAO

被引：72
作者：
RATNANINGSIH, E
DHARMSTHITI, S
KRISHNAPILLAI, V
MORGAN, A
SINCLAIR, M
HOLLOWAY, BW
机构：
[1] Department of Genetics, and Developmental Biology, Monash University, Clayton
来源：
JOURNAL OF GENERAL MICROBIOLOGY | 1990年 / 136卷
关键词：
D O I：
10.1099/00221287-136-12-2351
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome.
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页码：2351 / 2357
页数：7
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