PROLONGED INCUBATION IN CALCIUM-CHLORIDE IMPROVES THE COMPETENCE OF ESCHERICHIA-COLI-CELLS

被引：1328

作者：

DAGERT, M

EHRLICH, SD

机构：

[1] Laboratoire dEtude des Acides Nucléiques, Département de Microbiologie, Institut de Recherche en Biologie Moléculaire, 75005 Paris, 2, Place Jussieu

来源：

GENE | 1979年 / 6卷 / 01期

关键词：

ampicillin resista nce; recombinant DNA; Transformation with pBR322 plasmid DNA;

D O I：

10.1016/0378-1119(79)90082-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Escherichia coli cells are 4-6 times more transformable and 20-30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment. With 24-h-old competent cells we obtained routinely 2 · 107 transformants per μg of pBR322 DNA, and transformed over 20% of viable cells. © 1979.

引用

页码：23 / 28

页数：6

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