COMPARISON OF TESTS DESIGNED TO IDENTIFY STAPHYLOCOCCUS-AUREUS THERMOSTABLE NUCLEASE

被引：10
作者：
BRAKSTAD, OG
MAELAND, JA
CHESNEAU, O
机构：
[1] UNIV TRONDHEIM,SCH MED,DEPT MICROBIOL,TRONDHEIM,NORWAY
[2] INST PASTEUR,NATL REFERENCE CTR STAPHYLOCOCCI,STAPHYLOCOQUES & STREPTOCOQUES LAB,PARIS,FRANCE
来源：
APMIS | 1995年 / 103卷 / 03期
关键词：
STAPHYLOCOCCUS AUREUS; THERMOSTABLE NUCLEASE; MONOCLONAL ANTIBODIES; POLYMERASE CHAIN REACTION; THERMONUCLEASE TEST; ENZYME-LINKED IMMUNOSORBENT ASSAY;
D O I：
10.1111/j.1699-0463.1995.tb01098.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The thermonuclease test has been employed for same-day identification of Staphylococcus aureus in blood cultures and a seroinhibition test to confirm S. aureus thermonuclease (TNase) by using polyclonal anti-S. aureus TNase antiserum. However, strains of non-S. aureus staphylococci may produce TNases which are neutralized by the antiserum. This study evaluated alternative reagents and confirmatory tests for the S. aureus ens TNase. The tests included seroinhibition by a monoclonal antibody (MAb) against S. aureus TNase, MAb-based detection of the TNase in a sandwich ELISA, and a polymerase chain reaction for amplification of the nuc gene encoding the S. aureus TNase. All these tests discriminated between TNase produced by S. aureus and TNase produced by strains of the species S. caprae, S. carnosus, S. simulans, S. capitis, and S. intermedius. Specificity for the S. aureus TNase was confirmed by an inhibition ELISA for two out of three MAbs tested. Thus, the MAb and nuc-based tests will be useful to discriminate between TNases from S. aureus and non-S. aureus staphylococci.
引用
收藏
页码：219 / 224
页数：6
相关论文
共 25 条
[1]   THE ACCESSORY GENE REGULATOR (AGR) CONTROLS STAPHYLOCOCCUS-AUREUS VIRULENCE IN A MURINE ARTHRITIS MODEL [J].
ABDELNOUR, A ;
ARVIDSON, S ;
BREMELL, T ;
RYDEN, C ;
TARKOWSKI, A .
INFECTION AND IMMUNITY, 1993, 61 (09) :3879-3885
[2]  
BERGH K, 1986, ACTA PATH MICRO IM B, V94, P291
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   COMPARISON OF VARIOUS METHODS AND REAGENTS FOR SPECIES IDENTIFICATION OF STAPHYLOCOCCUS-AUREUS POSITIVE OR NEGATIVE FOR THE MECA GENE [J].
BRAKSTAD, OG ;
TVETEN, Y ;
NATO, F ;
FOURNIER, JM .
APMIS, 1993, 101 (08) :651-654
[5]   MULTIPLEX POLYMERASE CHAIN-REACTION FOR DETECTION OF GENES FOR STAPHYLOCOCCUS-AUREUS THERMONUCLEASE AND METHICILLIN RESISTANCE AND CORRELATION WITH OXACILLIN RESISTANCE [J].
BRAKSTAD, OG ;
MAELAND, JA ;
TVETEN, Y .
APMIS, 1993, 101 (09) :681-688
[6]   DETECTION OF STAPHYLOCOCCUS-AUREUS WITH BIOTINYLATED MONOCLONAL-ANTIBODIES DIRECTED AGAINST STAPHYLOCOCCAL TNASE COMPLEXED TO AVIDIN-PEROXIDASE IN A RAPID SANDWICH ENZYME-LINKED IMMUNOFILTRATION ASSAY (SELIFA) [J].
BRAKSTAD, OG ;
MAELAND, JA .
JOURNAL OF MEDICAL MICROBIOLOGY, 1993, 39 (02) :128-134
[7]   GENERATION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST STAPHYLOCOCCUS-AUREUS THERMONUCLEASE [J].
BRAKSTAD, OG ;
MAELAND, JA .
APMIS, 1989, 97 (02) :166-174
[8]   DIRECT IDENTIFICATION OF STAPHYLOCOCCUS-AUREUS IN BLOOD CULTURES BY DETECTION OF THE GENE ENCODING THE THERMOSTABLE NUCLEASE OR THE GENE-PRODUCT [J].
BRAKSTAD, OG ;
MAELAND, JA .
APMIS, 1995, 103 (03) :209-218
[9]   DETECTION OF STAPHYLOCOCCUS-AUREUS BY POLYMERASE CHAIN-REACTION AMPLIFICATION OF THE NUC GENE [J].
BRAKSTAD, OG ;
AASBAKK, K ;
MAELAND, JA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (07) :1654-1660
[10]   NUCLEOTIDE-SEQUENCE OF A NUC GENE ENCODING THE THERMONUCLEASE OF STAPHYLOCOCCUS-INTERMEDIUS [J].
CHESNEAU, O ;
ELSOLH, N .
NUCLEIC ACIDS RESEARCH, 1992, 20 (19) :5232-5232
123