PROOF OF INTRAGENIC RECOMBINATION IN PLASMODIUM-FALCIPARUM

被引：52

作者：

KERR, PJ ^{[1
]}

RANFORDCARTWRIGHT, LC ^{[1
]}

WALLIKER, D ^{[1
]}

机构：

[1] UNIV EDINBURGH,INST CELL ANIM & POPULAT BIOL,DIV BIOL SCI,EDINBURGH EH9 3JT,MIDLOTHIAN,SCOTLAND

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1994年 / 66卷 / 02期

基金：

英国医学研究理事会;

关键词：

PLASMODIUM FALCIPARUM; INTRAGENIC RECOMBINATION; MSP-1; GENE;

D O I：

10.1016/0166-6851(94)90151-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intragenic recombination in the merozoite surface protein MSP-1 of Plasmodium falciparum has been demonstrated in a cross between two cloned lines (3D7 and HB3) of this species. Following passage of a mixture of the clones through mosquitoes, uncloned progeny were examined by PCR for molecules containing sequences of both parent MSP-1-alleles. A recombinant molecule possessing both 3D7 and HB3 sequences has been obtained. Such molecules were not obtained from artificial mixtures of the blood forms of each clone. It is concluded that the novel allele was formed by a recombination event during meiosis of a hybrid 3D7/HB3 zygote.

引用

页码：241 / 248

页数：8

共 21 条

[11] 3RD FORM OF THE PRECURSOR TO THE MAJOR MEROZOITE SURFACE-ANTIGENS OF PLASMODIUM-FALCIPARUM [J].

PETERSON, MG ;

COPPEL, RL ;

MOLONEY, MB ;

KEMP, DJ .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (06) :2664-2667

[12] DIRECT SEQUENCING OF ENZYMATICALLY AMPLIFIED DNA OF ALLELES OF THE MEROZOITE SURFACE-ANTIGEN MSA-1 GENE FROM THE MALARIA PARASITE PLASMODIUM-FALCIPARUM [J].

RANFORDCARTWRIGHT, LC ;

BALFE, P ;

CARTER, R ;

WALLIKER, D .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 46 (01) :185-188

[13] FREQUENCY OF CROSS-FERTILIZATION IN THE HUMAN MALARIA PARASITE PLASMODIUM-FALCIPARUM [J].

RANFORDCARTWRIGHT, LC ;

BALFE, P ;

CARTER, R ;

WALLIKER, D .

PARASITOLOGY, 1993, 107 :11-18

[14] PRIMER-DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA-POLYMERASE [J].

SAIKI, RK ;

GELFAND, DH ;

STOFFEL, S ;

SCHARF, SJ ;

HIGUCHI, R ;

HORN, GT ;

MULLIS, KB ;

ERLICH, HA .

SCIENCE, 1988, 239 (4839) :487-491

[15]

Sambrook J., 1989, MOL CLONING LAB MANU

[16] MEROZOITE SURFACE-COAT PRECURSOR PROTEIN COMPLETELY PROTECTS AOTUS MONKEYS AGAINST PLASMODIUM-FALCIPARUM MALARIA [J].

SIDDIQUI, WA ;

TAM, LQ ;

KRAMER, KJ ;

HUI, GSN ;

CASE, SE ;

YAMAGA, KM ;

CHANG, SP ;

CHAN, EBT ;

KAN, SC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (09) :3014-3018

[17] HUMAN IMMUNODEFICIENCY VIRUS-INFECTED INDIVIDUALS CONTAIN PROVIRUS IN SMALL NUMBERS OF PERIPHERAL MONONUCLEAR-CELLS AND AT LOW COPY NUMBERS [J].

SIMMONDS, P ;

BALFE, P ;

PEUTHERER, JF ;

LUDLAM, CA ;

BISHOP, JO ;

BROWN, AJL .

JOURNAL OF VIROLOGY, 1990, 64 (02) :864-872

[18] ALLELIC DIMORPHISM IN A SURFACE-ANTIGEN GENE OF THE MALARIA PARASITE PLASMODIUM-FALCIPARUM [J].

TANABE, K ;

MACKAY, M ;

GOMAN, M ;

SCAIFE, JG .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 195 (02) :273-287

[19]

Walliker D., 1988, Molecular genetics of parasitic protozoa., P24

[20] GENETIC-ANALYSIS OF THE HUMAN MALARIA PARASITE PLASMODIUM-FALCIPARUM [J].

WALLIKER, D ;

QUAKYI, IA ;

WELLEMS, TE ;

MCCUTCHAN, TF ;

SZARFMAN, A ;

LONDON, WT ;

CORCORAN, LM ;

BURKOT, TR ;

CARTER, R .

SCIENCE, 1987, 236 (4809) :1661-1666

← 1 2 3 →