2 DISTINCT PATHWAYS OF SPECIFIC KILLING REVEALED BY PERFORIN MUTANT CYTOTOXIC T-LYMPHOCYTES

被引：285

作者：

KOJIMA, H

SHINOHARA, N

HANAOKA, S

SOMEYASHIROTA, Y

TAKAGAKI, Y

OHNO, H

SAITO, T

KATAYAMA, T

YAGITA, H

OKUMURA, K

SHINKAI, Y

ALT, FW

MATSUZAWA, A

YONEHARA, S

TAKAYAMA, H

机构：

[1] SCI UNIV TOKYO,FAC PHARMACEUT SCI,DEPT PATHOPHYSIOL,SHINJUKU KU,TOKYO 162,JAPAN

[2] MITSUBISHI KASEI INST LIFE SCI,MOLEC IMMUNOL LAB,MACHIDA,TOKYO 194,JAPAN

[3] CHIBA UNIV,SCH MED,CTR BIOMED SCI,DIV MOLEC GENET,CHUO KU,CHIBA 260,JAPAN

[4] JUNTENDO UNIV,SCH MED,DEPT IMMUNOL,BUNKYO KU,TOKYO 113,JAPAN

[5] CHILDRENS HOSP,HOWARD HUGHES MED INST,BOSTON,MA 02115

[6] UNIV TOKYO,INST MED SCI,LAB ANIM RES CTR,MINATO KU,TOKYO 108,JAPAN

[7] JT INC,PHARMACEUT BASIC RES LABS,KANAZAWA KU,YOKOHAMA,KANAGAWA 236,JAPAN

来源：

IMMUNITY | 1994年 / 1卷 / 05期

关键词：

D O I：

10.1016/1074-7613(94)90066-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To study the contribution of putative perforin-independent mechanism in the antigen-specific target destruction by cytotoxic T lymphocytes, CD8(+) CTL lines were established from spleen cells of chimeric mice produced by injecting perforin (-/-) embryonic stem cells into blastocysts of RAG-2(-/-) mice. When tested on normal concanavalin A blasts, these perforin-deficient cytotoxic T lymphocyte lines were found to be capable of inducing antigen-specific target cell lysis accompanied by DNA degradation. In contrast, with target cells carrying a mutation in Fas molecule, perforin-independent cytotoxicity was not detectable. These data not only confirmed the primary role of perforin but simultaneously revealed a major contribution of a perforin-independent Fas-mediated pathway in antigen-specific cytolysis.

引用

页码：357 / 364

页数：8

共 44 条

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